Total Internal Reflection Microscope

Hardware:

Nikon TE2000E Inverted Microscope with Perfect Focus with DIC, Phase, and Epi-fluorescence Optics

Nikon Laser TIRF Illuminator (laser lines: 440 nm, 488 nm, 514 nm, 561 nm)

Photometrics QuantEM EMCCD

Sutter Lambda 10-3 Emission Filter Wheel

Software:

NIS-Elements Advanced Research

Objectives:

Turret:
1. 10x /0.3 Air - Plan Apo (DIC N2)
2. 20x/0.75 Air - Plan Fluor ELWD (DIC N1)
3. 60x/1.45 Oil - Plan Apo TIRF (DIC N2)
4. 100x/1.49 Oil - Plan Apo TIRF (DIC N2)

Filters:

Emission Filter Wheel:
1. Closed
2. Open
3. 525/50m - (GFP, for use with 488/561)
4. 610/60m - (RFP, for use with 488/561)
5. 540/25m - (YFP, for use with 514/561)
6. 620/60m - (RFP, for use with 514/561)
7. 485/30m - (CFP, for use with 440/514)
8. 545/40m - (YFP, for use with 440/514)
9. Closed
10. Closed