Time lapse Microscope
Hardware:
Nikon TE2000E Inverted Microscope with Perfect Focus, DIC, phase contrast, and epi-fluorescence Optics
Photometrics Coolsnap HQ2 Camera
Applied Scientific Instrumentation MS-2000 Motorized Stage
Sutter Lambda LS Xenon Arc Lamp and Excitation Filter Wheel
Sutter Lambda 10-3 Emission Filter Wheel
In Vivo Scientific Incubator with humidification and CO2 control
Software:
NIS-Elements Advanced Research
Objectives:
1. 10x/0.3 Air - Plan Fluor (Ph1)
2. 20x/0.75 Air - Plan Apo (DIC N2)
3. 40x/0.75 Air - Plan Fluor (DIC N2)
4. 40x/1.3 Oil - Plan Fluor (DIC N2)
5. 60x/1.2 WI - Plan Apo (DIC N2)
6. 100x/1.4 Oil - Plan Apo (Ph3)
Filters:
Turret:
1. Sedat Quad
2. GFP/mRFP1
3. CFP/YFP
4. 505LP (roGPF)
5. DAPI/FITC/Rhodamine
6. Empty
Excitation Filters (Wheel A):
0. 390/22x Phil - roGFP
1. 485/15x Phil - roGFP
2. D350/50x - Sedat Quad
3. ET490/20x - Sedat Quad
4. ET555/25x - Sedat Quad
5. ET645/30x - Sedat Quad
6. S470/30x - GFP/mRFP1
7. S565/55x - GFP/mRFP1
8. ET430/24x - CFP/YFP
9. ET500/20x - CFP/YFP
Emission Filters (Wheel C):
0. 535/30m - Phil roGFP
1. Empty
2. ET455/50m - Sedat Quad
3. ET525/36m - Sedat Quad
4. ET605/52m - Sedat Quad
5. ET705/72m - Sedat Quad
6. S510/30m - GFP/mRFP1
7. S650/75m - GFP/mRFP1
8. ET470/24m - CFP/YFP
9. ET535/30m - CFP/YFP
