Swept-field Confocal Microscope

Hardware:

Nikon TE2000U Inverted Microscope with DIC, phase contrast, and epi-fluorescence optics

Nikon LiveScan Swept-Field Confocal

Available laser lines: 488nm

Photometrics Cascade II:512 Camera

Software:

NIS-Elements Advanced Research

Objectives:

1. 10x/0.30 Air - Plan Fluor (Ph1)
2. 20x/0.45 Air - Plan Fluor ELWD (Ph1) Collar
3. 40x/0.60 Air - Plan Fluor ELWD (DIC N1) Collar
4. 60x/1.45 Oil - Plan Apo TIRF (DIC N2) Collar
5. 60x/1.45 Oil - Plan Apo TIRF (DIC N2) Collar

Filters:

Turret:
1. Open
2. G2E/C
3. Open
4. GFP LP
5. YFP
6. DAPI/FITC/Rhodamine