Spinning Disk Confocal Microscope
Nikon Eclipse Ti-E Motorized Inverted Microscope
- Micromanager Open-Source Software
- Nikon integrated Perfect Focus System
- Yokogawa CSU22 Spinning Disk Confocal from Solamere Technology Group
- Coherent and Cobalt lasers: 405nm and 491nm (594nm and 640nm in the near future)
- Photometrics Evolve EMCCD Camera
- In Vivo Scientific Incubator
- Sutter Lambda 10-B Emission Filter Wheel (as fast as 40 ms for adjacent filters)
- Sutter Smart Shutters (8ms open/close time)
- Chroma Filters
| Objectives: | |||||||
|---|---|---|---|---|---|---|---|
| Objective | Resolution limit (nm) | Pixel Size (nm/px) | Depth of field (um) | Suggested Step Size (um) | Comments | ||
| 10x/0.30 na air Plan Fluor | 1017 | 654 | 14.4 | 5.76 | Phase 1 | ||
| 40x/0.95na air Plan Apo | 321 | 227.5 | 1.44 | 0.576 | DIC N2/40xI | ||
| 60x/1.20na WI Plan Apo VC | 254 | 151.7 | 0.9 | 0.36 | DIC N2/60xI | ||
| 60x/1.45na oil Plan Apo TIRF | 210 | 262 | 0.62 | 0.25 | DIC N2/60xII | ||
| 100x/1.40na oilPlan Apo VC | 218 | 91 | 0.66 | 0.25 | DIC N2/100xI | ||
| 100x/1.49 na oil Apo TIRF | 205 | 91 | 0.59 | 0.236 | DIC N2/100xI | ||
