Spectral Confocal Microscope

Hardware:

Nikon FN1 Upright Microscope

Nikon C1si Spectral Confocal

Available laser lines: Blue - 405nm, Green - 488nm, Red - 561nm

Software:

Objectives:

1. 10x/0.45 Air - Plan Apo (DIC N1)
2. 20x/0.75 Air - Plan Apo (DIC N2)
3. 40x/0.95 Air - Plan Fluor (DIC N2) Collar
4. 10x/0.30 Water - Plan Fluor (DIC N1)
5. 40x/0.80 Water - NIR Apo (DIC N2)
6. 60x/1.00 Water - NIR Apo (DIC N2)
7. 40x/1.30 Oil - Plan Fluor (DIC N2)
8. 60x/1.40 Oil - Plan Apo VC (DIC N2)
9. 100x/1.40 Oil - Plan Apo VC (DIC N2)

Filters:

Emission:
Blue - 450/35
Green - 525/50
Red - 605/60

Turret (For the Eyes):
1. UV2E/C (DAPI)
2. GFP LP
3. YFP
4. G2E/C (Rhodamine)
5. DAPI/FITC/Rhodamine
6. Confocal (empty)