Learning about Microscopy

The iBiology microscopy course is a comprehensive set of online lectures covering all aspects of modern light microscopy.

A family of websites, formerly maintained by Michael Davidson, are an excellent source of microscopy information:

Fluorescent Proteins

The NIC maintains a large library of fluorescent proteins for both mammalian expression and yeast tagging, available to UCSF investigators. To request plasmids, download the catalog and send Kurt Thorn an email with the name, box, and position number of the plasmids you want, along with a speedtype to charge $11.00 / plasmid to. If you are not a UCSF investigator, most of these plasmids are available at Addgene. Sequence files for these plasmids can be found here, as well as at Addgene.

We also have interactive graphs of fluorescent protein properties and of photoswitchable fluorescent protein properties to help you choose proteins for your experiment.

Other Microscopy Cores

There are a number of other microscopy cores at UCSF that may be useful for your research. They are listed below, with microscopes whose capabilities go beyond what is available at the NIC called out in parentheses.

Other core instrumentation can be found at the Cores search page.

Experimental Design

Protocols

More specialized protocols and detailed information are available on the NIC Wiki.