Learning about Microscopy
The iBiology microscopy course is a comprehensive set of online lectures covering all aspects of modern light microscopy.
A family of websites, formerly maintained by Michael Davidson, are an excellent source of microscopy information:
- Molecular Expressions
- Nikon Microscopy U
- Zeiss MicroImaging Campus
- Olympus Microscopy Resource Center
The NIC maintains a large library of fluorescent proteins for both mammalian expression and yeast tagging, available to UCSF investigators. To request plasmids, download the catalog and send DeLaine Larsen an email with the name, box, and position number of the plasmids you want, along with a speedtype to charge $11.00 / plasmid to. If you are not a UCSF investigator, most of these plasmids are available at Addgene. Sequence files for these plasmids can be found here, as well as at Addgene.
Other Microscopy Cores
There are a number of other microscopy cores at UCSF that may be useful for your research. They are listed below, with microscopes whose capabilities go beyond what is available at the NIC called out in parentheses.
- Biological Imaging Development Center (2-photon microscopy)
- Center for Advanced Multiphoton Microscopy (2-photon microscopy)
- DRC Microscopy Core
- Gladstone Histology and Light Microscopy Core (Zeiss 880 confocal with Airyscan)
- Laboratory for Cell Analysis (Zeiss 780 confocal with 2-photon and spectral imaging)
Other core instrumentation can be found at the Cores search page.
- Choosing multiwell plates and dishes for imaging
- Dealing with imaging data – issues to consider before acquiring large data sets.
More specialized protocols and detailed information are available on the NIC Wiki.