Capabilities

  • Brightfield, phase contrast, and DIC imaging
  • 4-color fluorescence imaging
  • Live cell imaging
  • Automated time lapse imaging
  • Automated multiwell plate imaging

Equipment

  • Nikon Ti Microscope
  • Hamamatsu Flash4.0 camera
  • Sutter XL lamp
  • Sutter excitation and emission filter wheels

Lenses

Installed on the microscope:

  • 10x / 0.3 Plan Fluor
  • 20x / 0.75 Plan Apo VC
  • 40x / 0.75 Plan Fluor

Other lenses can be signed out from our large collection of Nikon objectives.

Filters

Example DyeExcitation FilterDichroicEmission Filter
DAPI387/11xFF410/504/582/669440/40m
FITC485/20x FF410/504/582/669525/30m
Cy3560/25x FF410/504/582/669607/36m
Cy5650/13xFF410/504/582/669684/24m
GFPET470/40x59022bsET525/50m
mCherryET572/35x59022bsET632/60m
CFPET430/24x69008bsET470/24m
YFPET500/20x 69008bsET535/30m
Fura-2ET340xT400lpET510/80m
Fura-2ET380xT400lpET510/80m

Additional information about the Time Lapse microscope can be found on the NIC wiki.