Capabilities

  • 3D confocal imaging
  • 4-color fluorescence imaging
  • Live cell time lapse imaging

Equipment

  • Nikon Ti Microscope
  • Yokogawa CSU-22 Spinning Disk Confocal
  • Custom laser launch (100 mW at 405, 488, 561, and 640 nm)
  • Photometrics Evolve Delta EMCCD camera
  • Sutter emission filter wheel
  • Prior motorized XY stage with Z piezo

Lenses

Installed on the microscope:

  • Plan Fluor 10x /0.3
  • Plan Apo 40x/0.95 Corr
  • Plan Apo VC 60x/1.2 WI
  • Plan Apo VC 60x/1.4 Oil
  • Plan Apo VC 100x/1.4 Oil
  • Apo TIRF 100x/1.49 Oil

Other lenses can be signed out from our large collection of Nikon objectives.

Filters

Example DyeLaserEmission Filter
DAPI405 nmET460/50m
FITC488 nmET525/36m
Cy3561 nmET610/60m
Alexa 594561 nmET645/65m
Cy5640 nmET700/75m
MulticolorAnyzET405/488/561/635m

Additional information about the spinning disk confocal can be found on the NIC wiki.