Capabilities
- Super-resolution imaging by structured illumination (up to 120 nm in XY, 350 nm in Z)
- 4-color fluorescence imaging
Equipment
- Nikon Ti Microscope with N-SIM
- Nikon 4-line laser launch
- Nikon motorized stage
- Andor DU-897 EMCCD
Lenses
Installed on the microscope:
- 60x / 1.27 Plan Apo IR WI
- 100x / 1.49 Apo TIRF
Filters
- 405 nm excitation for DAPI and similar dyes
- 488 nm excitation for FITC / GFP and similar dyes
- 561 nm excitation for Cy3 / RFP and similar dyes
- 640 nm excitation for Cy5 and similar dyes
Additional information about the N-SIM can be found on the NIC wiki.
