Capabilities

  • Super-resolution imaging by structured illumination (up to 120 nm in XY, 350 nm in Z)
  • 4-color fluorescence imaging

Equipment

  • Nikon Ti Microscope with N-SIM
  • Nikon 4-line laser launch
  • Nikon motorized stage
  • Andor DU-897 EMCCD

Lenses

Installed on the microscope:

  • 60x / 1.27 Plan Apo IR WI
  • 100x / 1.49 Apo TIRF

Filters

  • 405 nm excitation for DAPI and similar dyes
  • 488 nm excitation for FITC / GFP and similar dyes
  • 561 nm excitation for Cy3 / RFP and similar dyes
  • 640 nm excitation for Cy5 and similar dyes

Additional information about the N-SIM can be found on the NIC wiki.