Capabilities

  • Light Sheet imaging of cleared tissues up to 2 cm on a side
  • 4-color fluorescence imaging
  • 3D imaging
  • Automated image stitching

Equipment

  • Nikon AZ-100 microscope
  • Vortran 4-line laser launch
  • Andor Zyla 5.5 camera
  • ASI motorized stage
  • Custom light sheet optics

Lenses

Installed on the microscope:

  • 1x / 0.1 AZ Plan Apo
  • 2x / 0.2 AZ Plan Apo
  • 5x /0.5 AZ Plan Apo

Filters

Example DyeLaserEmission Filter
DAPI405 nmET455/50m
FITC488 nmET535/36m
Cy3561 nmET605/52m
Cy5640 nmET705/72m

Additional information about the 6D / High Throughput microscope can be found on the NIC wiki.