Capabilities

  • Color brightfield imaging
  • 5-color fluorescence imaging
  • Automated image stitching

Equipment

  • Nikon Ti Microscope
  • Nikon DS-Qi2 monochrome and DS-Ri2 color cameras
  • Sutter Lambda LS-2 xenon arc lamp
  • Sutter excitation and emission filter wheels

Lenses

Installed on the microscope:

  • 4x / 0.2 Plan Apo λ
  • 10x / 0.45 Plan Apo
  • 20x / 0.75 Plan Apo VC

Other lenses can be signed out from our large collection of Nikon objectives.

Filters

Example DyeExcitation FilterDichroicEmission Filter
DAPI387/11xFF408/504/581/667/762440/40m
FITC485/20x FF408/504/581/667/762525/30m
Cy3560/25x FF408/504/581/667/762607/36m
Cy5650/13xFF408/504/581/667/762684/24m
Cy7740/13xFF408/504/581/667/762809/81m
GFPET470/40x59022bsET525/50m
mCherryET572/35x59022bsET632/60m
CFPET430/24x69008bsET470/24m
YFPET500/20x 69008bsET535/30m

Additional information about the 6D / High Throughput microscope can be found on the NIC wiki.