EQUIPMENT
| - Image Acquisition Stations - |
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A spinning disk confocal microscope for
capturing images of thin optical sections of the sample.Click for more information. B - Swept-field confocal
This confocal microscope is designed for maximum flexibility allowing the user the choice to capture images at very high resolution or very quickly. Rather than scanning a single point over the specimen, either a slit or an array of pinholes is scanned over the specimen.Click for more information. C - TIRF
"Total Internal Reflection Fluorescence" for
high-sensitivity imaging approximately 100 nm adjacent to the coverslip with Perfect Focus to maintain imaging plane.Click for more information. D - Time lapse
37C chamber incubated time lapse imaging using Nikon
Perfect Focus to maintain focus during multi-day experiments.Click for more information. E - 6D High throughput
Motorized stage microscope for automated
imaging of microtiter platesClick for more information. F - Spectral confocal
Wide-band high-resolution spectral imaging
microscope, with anesthesia capability for live animal imaging.Click for more information.
- Image Analysis -
Six PC computer workstations will be available for off-line image analysis. Each computer will be equipped with Metamorph image analysis software and NIS Elements. Data may be accessed through the NIC server, CD, or DVD. These computers can also be used to download data from the NIC server to CD or DVD.
- Data Storage -
The NIC@QB3 does not offer long-term data storage. Storage of data on the NIC@QB3 computer hard drives is not allowed; the hard drives of each computer in the NIC@QB3 are purged weekly. We have arranged the following short-term storage options to allow users to transfer data out of the NIC@QB3:
Data may temporarily be stored on the NIC server. Files are automatically and permanently purged from the NIC server after a period of one month. To access the NIC server, you must have an account in the UCSF Active Directory system. The easiest way to do so is to get an @ucsf.edu email address. If you do not have one, one can be obtained at help.ucsf.edu. The file server has two 1.5 TB disks, located at \\nicdata.ucsf.edu\data0 and \\nicdata.ucsf.edu\data1. You will need to logon with the same username and password you use to access your @ucsf.edu email.
Alternatively, users can copy their data off of the microscope workstations via CD/DVD or with a USB drive.
- Sample Handling -
A Biosafety Hood is available for sample preparation in the NIC@QB3. We have a tissue culture incubator for short-term storage of your specimens while you are acquiring images in the NIC@QB3 on order. You must file a or amend an existing BUA listing the NIC lab space before bringing BSL2 level samples into the NIC.
