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TIRF Microscope
Documentation
Optics
Objectives
Plan Fluor 10x /0.3 (Ph1) 1600 nm/pixel
Plan Apo 20x/0.75 (DIC N2 / 20X) 785 nm/pixel
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Plan Apo VC 60XA/1.20 Water
Plan Apo lambda 100x/1.45 TIRF Oil 157 nm/pixel
Apo TIRF 100x/1.49 Oil (DIC N2 / 100X I) 157 nm/pixel
Filter Turret 1 (FL1)
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Analyzer
Quad N-STORM
DAPI/FITC/Rhodamine Triple (for eyepiece viewing with arc lamp)
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Emission Wheel
ET455/50m
ET525/50m
ET600/60m
ET705/72m
ZET488/561/640m (triple bandpass)
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Blocker
Blocker
Blocker
Hardware
Nikon Ti-E Microscope
Sutter emission wheel and 10-B
Nikon motorized stage with piezo Z insert
Evolve on left port
Andor DU897 on right port with STORM lens
Agilent MLC400 with NIDAQ interface
Brightfield shutter controlled by Sutter Lambda SC through Ti
Interface Requirements
Firmware
Ti: Combination I
TiControl: 4.3.2
PC
Software
Monthly Quality Control Info