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TIRF Microscope

Optics

Objectives
  1. Plan Fluor 10x /0.3 (Ph1) 1600 nm/pixel
  2. Plan Apo 20x/0.75 (DIC N2 / 20X) 785 nm/pixel
  3. Empty
  4. Plan Apo VC 60XA/1.20 Water
  5. Plan Apo lambda 100x/1.45 TIRF Oil 157 nm/pixel
  6. Apo TIRF 100x/1.49 Oil (DIC N2 / 100X I) 157 nm/pixel
Filter Turret 1 (FL1)
  1. Empty
  2. Analyzer
  3. Quad N-STORM
  4. DAPI/FITC/Rhodamine Triple (for eyepiece viewing with arc lamp)
  5. Empty
  6. Empty
Emission Wheel
  1. Empty
  2. ET525/50m
  3. ET600/60m
  4. ET705/72m
  5. Empty
  6. Empty
  7. Empty
  8. Empty
  9. Empty
  10. Empty

Hardware

  • Nikon Ti-E Microscope
  • Nikon Emission wheel
  • Nikon motorized stage with piezo Z insert
  • QuantEM on left port and Evolve on rear port
  • Nikon LUSU laser controller
  • Brightfield shutter controlled by Sutter Lambda SC through Ti

Interface Requirements

  • 1 serial port for LUSU
  • 2 Firewire for QuantEMs
  • 3 USB connectors

Firmware

  • Ti: Combination I
  • TiControl: 4.30

PC

  • Pentium D 3.2 GHz
  • 4 GB RAM
  • 64 bit Windows 7
  • NVidia 7600GT
  • 480GB Hard drive (RAID 1)

Software

  • NIS-Elements 3.22 (Build 710)
/var/www/html/dokuwiki/data/attic/tirf.1335464264.txt.gz · Last modified: 2012/04/26 06:17 (external edit)