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TIRF Microscope

Optics

Objectives
  1. Plan Fluor 10x /0.3 (Ph1) 1600 nm/pixel
  2. Plan Apo 20x/0.75 (DIC N2 / 20X) 785 nm/pixel
  3. Empty
  4. Empty
  5. Plan Apo 60x/1.45 TIRF Oil (DIC N2 / 60X II) 262 nm/pixel
  6. Plan Apo 100x/1.49 TIRF Oil (DIC N2 / 100X I) 157 nm/pixel
Filter Turret 1 (FL1, Top)
  1. Empty
  2. 440/514 High SNR TIRF (CFP/YFP)
  3. 488/561 High SNR TIRF (GFP/RFP)
  4. 514/561 TIRF (YFP/RFP)
  5. 488/561 FRAP (YFP/RFP)
  6. D/F/R
Filter Turret 2 (FL2, Bottom)
  1. Analyzer
  2. Empty
  3. Empty
  4. Empty
  5. Empty
  6. Empty
Emission Wheel
  1. ET480/40M (CFP, High SNR)
  2. ET540/40M (YFP, High SNR)
  3. ET525/50m (GFP High SNR, for use with 488/561)
  4. ET600/50m (RFP High SNR, for use with 488/561)
  5. 540/25m (YFP, for use with 514/561)
  6. 620/60m (RFP, for use with 514/561)
  7. Empty
  8. Empty

Hardware

  • Nikon Ti-E Microscope
  • Nikon Emission wheel
  • QuantEM on left port and QuantEM on rear port
  • Nikon LUSU laser controller
  • Brightfield shutter controlled by Sutter Lambda SC through Ti

Interface Requirements

  • 1 serial port for LUSU
  • 2 Firewire for QuantEMs
  • 3 USB connectors

Firmware

  • Ti: Combination I
  • TiControl: 4.30

PC

  • Pentium D 3.2 GHz
  • 4 GB RAM
  • 64 bit Windows 7
  • NVidia 7600GT
  • 480GB Hard drive (RAID 1)

Software

  • NIS-Elements 3.22 (Build 710)
/var/www/html/dokuwiki/data/attic/tirf.1303947524.txt.gz · Last modified: 2011/04/27 11:38 (external edit)