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tirf [2011/04/12 12:11]
kthorn created
tirf [2017/11/03 08:57]
dlarsen
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 ==== TIRF Microscope ==== ==== TIRF Microscope ====
 +
 +=== Documentation ===
 +{{:​m605_e1_n-storm_simple_operation_security_.pdf|Nikon STORM manual}}
 +
 +=== Optics ===
 +
 +== Objectives ==
 +
 +  - Plan Fluor 10x /0.3 (Ph1) 1600 nm/pixel
 +  - Plan Apo 20x/​0.75 (DIC N2 / 20X) 785 nm/pixel
 +  - Empty
 +  - Plan Apo VC 60XA/1.20 Water
 +  - Plan Apo lambda 100x/1.45 TIRF Oil 157 nm/pixel
 +  - Apo TIRF 100x/1.49 Oil (DIC N2 / 100X I) 157 nm/pixel
 +
 +== Filter Turret 1 (FL1) ==
 +  - Empty
 +  - Analyzer
 +  - Quad N-STORM
 +  - DAPI/​FITC/​Rhodamine Triple (for eyepiece viewing with arc lamp)
 +  - Empty
 +  - Empty
 +
 +== Emission Wheel == 
 +  - ET455/50m
 +  - ET525/50m
 +  - ET600/60m
 +  - ET705/72m
 +  - ZET488/​561/​640m (triple bandpass)
 +  - ZET405/​488/​561m (triple bandpass)
 +  - Empty
 +  - Blocker
 +  - Blocker
 +  - Blocker
 +
  
 === Hardware === === Hardware ===
  
   * Nikon Ti-E Microscope   * Nikon Ti-E Microscope
-  * Nikon Emission ​wheel +  * Sutter emission ​wheel and 10-B 
-  * QuantEM ​on left port and QuantEM ​on rear port +  * Nikon motorized stage with piezo Z insert 
-  * Nikon LUSU laser controller+  * Evolve ​on left port 
 +  * Andor DU897 on right port with STORM lens 
 +  * Agilent MLC400 with NIDAQ interface
   * Brightfield shutter controlled by Sutter Lambda SC through Ti   * Brightfield shutter controlled by Sutter Lambda SC through Ti
  
 === Interface Requirements === === Interface Requirements ===
  
-  * 1 serial port for LUSU 
-  * 2 Firewire for QuantEMs 
   * 3 USB connectors   * 3 USB connectors
  
 === Firmware === === Firmware ===
   * Ti: Combination I   * Ti: Combination I
-  * TiControl: 4.30+  * TiControl: 4.3.2
  
 === PC === === PC ===
-  * Pentium D 3.2 GHz +  * Xeon W3670 (3.2GHz) 
-  * GB RAM +  * 24 GB RAM 
-  * 32 bit XP +  * 64 bit Windows 7 
-  * NVidia ​7600GT +  * NVidia ​Quadro 600 
-  * 480GB Hard drive (RAID 1)+  * 280 GB C: Hard drive 
 +  * 1TB D: Hard drive (RAID 1)
  
 === Software === === Software ===
-  * NIS-Elements ​3.22 (Build 710)+  * NIS-Elements ​4.
  
-=== Optics ​=== +=== Monthly Quality Control Info  ​===
- +
-== Objectives ​==+
  
-  - Plan Fluor 10x /0.3 (Ph1) 1.6 m/pixel 
-  - Plan Apo20x/​0.75 (DIC N2 / 20X) 785 nm/pixel 
-  - Plan Apo 60x/1.45 TIRF Oil (DIC N2 / 60X II) 262 nm/pixel 
-  - Plan Apo100x/​1.49 TIRF Oil (DIC N2 / 100X I) 157 nm/pixel 
- 
-== Filter Turret 1 (FL1, Top) == 
-  - Empty 
-  - 440/514 High SNR TIRF (CFP/YFP) 
-  - 488/561 High SNR TIRF (GFP/RFP) 
-  - 514/561 TIRF  (YFP/RFP) 
-  - 488/561 FRAP (YFP/RFP) 
-  - D/F/R 
- 
-== Filter Turret 2 (FL2, Bottom) == 
-  - Analyzer 
-  - Empty 
-  - Empty 
-  - Empty 
-  - Empty 
-  - Empty 
- 
-== Excitation Wheel ==  
-  - 390/​22x Phil roGFP 
-  - 485/​15x Phil roGFP 
-  - ET490/​20x Sedat Quad 
-  - ET555/​25x Sedat Quad  
-  - ET645/​30x Sedat Quad 
-  - ET470/​40x GFP/​mCherry ET 
-  - ET572/​35x GFP/​mCherry ET 
-  - ET430/​24x CFP/​YFP 
-  - ET500/​20x CFP/​YFP 
- 
-== Emission Wheel ==  
-  - 535/​30m Phil roGFP 
-  - 535/​30m Phil roGFP 
-  - ET455/​50m Sedat Quad 
-  - ET525/​36m Sedat Quad 
-  - ET605/​52m Sedat Quad 
-  - ET705/​72m Sedat Quad 
-  - ET525/​50m GFP/​mCherry ET 
-  - ET632/​60m GFP/​mCherry ET 
-  - ET470/​24m CFP/​YFP 
-  - ET535/​30m CFP/​YFP 
  
 +  * [[TIRF Laser Power|TIRF Laser Power]]
/var/www/html/dokuwiki/data/pages/tirf.txt · Last modified: 2017/11/03 08:57 by dlarsen