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tirf

TIRF Microscope

Documentation

Optics

Objectives
  1. Plan Fluor 10x /0.3 (Ph1) 1600 nm/pixel
  2. Plan Apo 20x/0.75 (DIC N2 / 20X) 785 nm/pixel
  3. Empty
  4. Plan Apo VC 60XA/1.20 Water
  5. Plan Apo lambda 100x/1.45 TIRF Oil 157 nm/pixel
  6. Apo TIRF 100x/1.49 Oil (DIC N2 / 100X I) 157 nm/pixel
Filter Turret 1 (FL1)
  1. Empty
  2. Analyzer
  3. Quad N-STORM
  4. DAPI/FITC/Rhodamine Triple (for eyepiece viewing with arc lamp)
  5. Empty
  6. Empty
Emission Wheel
  1. ET455/50m
  2. ET525/50m
  3. ET600/60m
  4. ET705/72m
  5. ZET488/561/640m (triple bandpass)
  6. Empty
  7. Empty
  8. Blocker
  9. Blocker
  10. Blocker

Hardware

  • Nikon Ti-E Microscope
  • Sutter emission wheel and 10-B
  • Nikon motorized stage with piezo Z insert
  • Evolve on left port
  • Andor DU897 on right port with STORM lens
  • Agilent MLC400 with NIDAQ interface
  • Brightfield shutter controlled by Sutter Lambda SC through Ti

Interface Requirements

  • 3 USB connectors

Firmware

  • Ti: Combination I
  • TiControl: 4.3.2

PC

  • Xeon W3670 (3.2GHz)
  • 24 GB RAM
  • 64 bit Windows 7
  • NVidia Quadro 600
  • 280 GB C: Hard drive
  • 1TB D: Hard drive (RAID 1)

Software

  • NIS-Elements 4.5

Monthly Quality Control Info

/var/www/html/dokuwiki/data/pages/tirf.txt · Last modified: 2016/10/04 13:37 by kthorn