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time_lapse

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Time Lapse Microscope

Optics

Objectives
  1. Plan Fluor 10x/0.3 (Ph1) 654nm/pixel
  2. Plan Apo 20x/0.75 (DIC N2 / 20X) 327nm/pixel
  3. Plan Fluor 40x/0.75 (DIC N2 / 40X I) 164nm/pixel
  4. Plan Fluor 40x/1.3 Oil (DIC N2/ 40X II) 164nm/pixel
  5. Plan Apo 60x/1.2 WI (DIC N2/ 60X II) 109nm/pixel
  6. Plan Apo 100x/1.4 Oil (Ph3) 65.4nm/pixel
Filter Turret
  1. Semrock DAPI/FITC/TRITC/Cy5 Sedat Quad
  2. GFP/mRFP1
  3. CFP/YFP
  4. FURA2 (Emission Filter within Cube)
  5. D/F/R
  6. Analyzer
Excitation from Lambda 421
LED wavelengthFilter in front of LED
340 nmFF01-340/26-25-STR
380 nmFF01-389/38-25-STR
440 nmFF01-445/40-25-STR
480 nmFF01-480/40-25-STR
530 nmFF01-520/44-25-STR
561 nmFF01-562/40-25-STR
630 nmFF01-630/38-25-STR
Emission Wheel
  1. Fura2 emission filter
  2. Empty
  3. 440/11m Semrock Sedat Quad
  4. 525/30m Semrock Sedat Quad
  5. 607/36m Semrock Sedat Quad
  6. 684/24m Semrock Sedat Quad
  7. ET525/50m GFP/mCherry ET (89021 Em2)
  8. ET632/60m GFP/mCherry ET (89021 Em1)
  9. ET470/24m CFP/YFP (89002 Em2)
  10. ET535/30m CFP/YFP (89002 Em1)

Hardware

  • Nikon Ti-E Microscope
  • External Phase Unit
  • Nikon motorized stage
  • Sutter Lambda 421 LED light source
  • Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM5, 128k Baud Rate)
  • Coolsnap HQ2 (Left port)
  • Hamamatsu Flash 4.0 (Right port)
  • In Vivo Scientific incubator and CO2 control

Interface Requirements

  • 1 serial port for 10-3
  • Firewire for Coolsnap HQ2
  • 3 USB connectors
  • 10-B XL: COM5 (USB)
  • 10-3: COM2

Firmware

  • Ti: Combination K
  • TiControl: 4.32

PC

  • NIC-HighSpeed2
  • Intel Core i7 4770K (3.50 GHz)
  • 32 GB RAM
  • 64bit Windows 7
  • 238 GB SSD Hard drive
  • 1 TB SSD Hard drive array (Raid 0)
  • 2 TB Hard drive (RAID 1)
  • NVIDA GeForce GTX 760
  • Active Silicon FireBird Card

Software

  • NIS-Elements 4.2 (Build 982)
Excitation Wheel (filters if you used Lambda XL lamp for earlier experiments)
  1. ET340x Fura-2 (79000 Ex1)
  2. ET380x Fura-2 (79000 Ex2)
  3. 387/11x Semrock Sedat Quad
  4. 485/20x Semrock Sedat Quad
  5. 560/25x Semrock Sedat Quad
  6. 650/13x Semrock Sedat Quad
  7. ET470/40x GFP/mCherry ET (89021 Ex2)
  8. ET572/35x GFP/mCherry ET (89021 Ex1)
  9. ET430/24x CFP/YFP (89002 Ex1)
  10. ET500/20x CFP/YFP (89002 Ex2)
/var/www/html/dokuwiki/data/attic/time_lapse.1511817830.txt.gz · Last modified: 2017/11/27 13:23 by dlarsen

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