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time_lapse [2014/03/14 13:10]
dlarsen
time_lapse [2019/01/09 13:18] (current)
dlarsen
Line 20: Line 20:
   - Analyzer   - Analyzer
  
-== Excitation ​Wheel ==  +== Excitation ​from Lambda 421 ==  
-  - ET340x ​     Fura-2 (79000 Ex1) +^LED wavelength^Filter in front of LED^ 
-  ET380x ​     Fura-2 (79000 Ex2) +|340 nm|FF01-340/26-25-STR| 
-  ​387/​11x ​    ​Semrock Sedat Quad  +|380 nm|FF01-389/38-25-STR| 
-  485/20x Semrock Sedat Quad  +|440 nm|FF01-445/40-25-STR| 
-  ​560/​25x Semrock Sedat Quad  +|480 nm|FF01-480/40-25-STR| 
-  650/13x Semrock Sedat Quad  +|530 nm|FF01-520/44-25-STR| 
-  ET470/​40x GFP/mCherry ET (89021 Ex2) +|561 nm|FF01-562/40-25-STR| 
-  ET572/​35x GFP/mCherry ET (89021 Ex1) +|630 nm|FF01-630/38-25-STR|
-  ET430/​24x CFP/YFP (89002 Ex1) +
-  ET500/20x CFP/YFP (89002 Ex2)+
  
 == Emission Wheel ==  == Emission Wheel == 
Line 49: Line 47:
   * External Phase Unit   * External Phase Unit
   * Nikon motorized stage   * Nikon motorized stage
-  * Sutter Lambda ​XL lamp with filter wheel and shutter (COM4, 128k Baud Rate)+  * Sutter Lambda ​421 LED light source ​with NIDAQ interface
   * Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM5, 128k Baud Rate)   * Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM5, 128k Baud Rate)
-  * Coolsnap HQ2 (Left port) 
   * Hamamatsu Flash 4.0 (Right port)   * Hamamatsu Flash 4.0 (Right port)
-  * In Vivo Scientific ​incubator and CO2 control+  * OkoLab ​incubator and CO2 control
  
 === Interface Requirements === === Interface Requirements ===
  
   * 1 serial port for 10-3   * 1 serial port for 10-3
-  * Firewire for Coolsnap HQ2 
   * 3 USB connectors   * 3 USB connectors
  
Line 81: Line 77:
 === Software === === Software ===
   * NIS-Elements 4.2 (Build 982)   * NIS-Elements 4.2 (Build 982)
 +
 +== Excitation Wheel (filters if you used Lambda XL lamp for earlier experiments) == 
 +  - ET340x ​     Fura-2 (79000 Ex1)
 +  - ET380x ​     Fura-2 (79000 Ex2)
 +  - 387/​11x ​    ​Semrock Sedat Quad 
 +  - 485/​20x Semrock Sedat Quad 
 +  - 560/​25x Semrock Sedat Quad
 +  - 650/​13x Semrock Sedat Quad 
 +  - ET470/​40x GFP/​mCherry ET (89021 Ex2)
 +  - ET572/​35x GFP/​mCherry ET (89021 Ex1)
 +  - ET430/​24x CFP/​YFP (89002 Ex1)
 +  - ET500/​20x CFP/​YFP (89002 Ex2)
  
/var/www/html/dokuwiki/data/attic/time_lapse.1394827819.txt.gz · Last modified: 2014/03/14 13:10 by dlarsen