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time_lapse [2014/01/09 11:48]
dlarsen
time_lapse [2019/01/09 13:18] (current)
dlarsen
Line 13: Line 13:
  
 == Filter Turret == == Filter Turret ==
-  - Sedat Quad+  - Semrock DAPI/​FITC/​TRITC/​Cy5 ​Sedat Quad
   - GFP/mRFP1   - GFP/mRFP1
   - CFP/YFP   - CFP/YFP
Line 20: Line 20:
   - Analyzer   - Analyzer
  
-== Excitation ​Wheel ==  +== Excitation ​from Lambda 421 ==  
-  - ET340x ​     Fura-2 (79000 Ex1) +^LED wavelength^Filter in front of LED^ 
-  ET380x ​     Fura-2 (79000 Ex2) +|340 nm|FF01-340/26-25-STR| 
-  ​ET402/​15x ​  Sedat Quad (89000 Ex4) +|380 nm|FF01-389/38-25-STR| 
-  ET490/20x Sedat Quad (89000 Ex3) +|440 nm|FF01-445/40-25-STR| 
-  ​ET555/​25x Sedat Quad (89000 Ex1)  +|480 nm|FF01-480/40-25-STR| 
-  ET645/30x Sedat Quad (89000 Ex2) +|530 nm|FF01-520/44-25-STR| 
-  ET470/​40x GFP/mCherry ET (89021 Ex2) +|561 nm|FF01-562/40-25-STR| 
-  ET572/​35x GFP/mCherry ET (89021 Ex1) +|630 nm|FF01-630/38-25-STR|
-  ET430/​24x CFP/YFP (89002 Ex1) +
-  ET500/20x CFP/YFP (89002 Ex2)+
  
 == Emission Wheel ==  == Emission Wheel == 
 +  - Fura2 emission filter
   - Empty   - Empty
-  - Empty +  - 440/11m Semrock ​Sedat Quad 
-  - ET455/50m Sedat Quad (89000 Em3) +  - 525/30m Semrock ​Sedat Quad  
-  - ET525/36m Sedat Quad (89000 Em2) +  - 607/36m Semrock ​Sedat Quad  
-  - ET605/52m Sedat Quad (89000 Em1) +  - 684/24m Semrock ​Sedat Quad 
-  - ET705/72m Sedat Quad (89000 Em4)+
   - ET525/​50m GFP/​mCherry ET (89021 Em2)   - ET525/​50m GFP/​mCherry ET (89021 Em2)
   - ET632/​60m GFP/​mCherry ET (89021 Em1)   - ET632/​60m GFP/​mCherry ET (89021 Em1)
Line 49: Line 47:
   * External Phase Unit   * External Phase Unit
   * Nikon motorized stage   * Nikon motorized stage
-  * Sutter Lambda ​XL lamp with filter wheel and shutter (COM4, 128k Baud Rate)+  * Sutter Lambda ​421 LED light source ​with NIDAQ interface
   * Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM5, 128k Baud Rate)   * Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM5, 128k Baud Rate)
-  * Coolsnap HQ2 (Left port) 
   * Hamamatsu Flash 4.0 (Right port)   * Hamamatsu Flash 4.0 (Right port)
-  * In Vivo Scientific ​incubator and CO2 control+  * OkoLab ​incubator and CO2 control
  
 === Interface Requirements === === Interface Requirements ===
  
   * 1 serial port for 10-3   * 1 serial port for 10-3
-  * Firewire for Coolsnap HQ2 
   * 3 USB connectors   * 3 USB connectors
  
Line 80: Line 76:
  
 === Software === === Software ===
-  * NIS-Elements 4.2 (Build ​967)+  * NIS-Elements 4.2 (Build ​982) 
 + 
 +== Excitation Wheel (filters if you used Lambda XL lamp for earlier experiments) ==  
 +  - ET340x ​     Fura-2 (79000 Ex1) 
 +  - ET380x ​     Fura-2 (79000 Ex2) 
 +  - 387/​11x ​    ​Semrock Sedat Quad  
 +  - 485/​20x Semrock Sedat Quad  
 +  - 560/​25x Semrock Sedat Quad  
 +  - 650/​13x Semrock Sedat Quad  
 +  - ET470/​40x GFP/​mCherry ET (89021 Ex2) 
 +  - ET572/​35x GFP/​mCherry ET (89021 Ex1) 
 +  - ET430/​24x CFP/​YFP (89002 Ex1) 
 +  - ET500/​20x CFP/​YFP (89002 Ex2)
  
/var/www/html/dokuwiki/data/attic/time_lapse.1389296926.txt.gz · Last modified: 2014/01/09 11:48 by dlarsen