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Time Lapse Microscope
Optics
Objectives
Plan Fluor 10x/0.3 (Ph1) 654nm/pixel
Plan Apo 20x/0.75 (DIC N2 / 20X) 327nm/pixel
Plan Fluor 40x/0.75 (DIC N2 / 40X I) 164nm/pixel
Plan Fluor 40x/1.3 Oil (DIC N2/ 40X II) 164nm/pixel
Plan Apo 60x/1.2 WI (DIC N2/ 60X II) 109nm/pixel
Plan Apo 100x/1.4 Oil (Ph3) 65.4nm/pixel
Filter Turret
Sedat Quad
GFP/mRFP1
CFP/YFP
FURA2 (Emission Filter within Cube)
D/F/R
Analyzer
Excitation Wheel
ET340x Fura-2 (79000 Ex1)
ET380x Fura-2 (79000 Ex2)
ET402/15x Sedat Quad (89000 Ex4)
ET490/20x Sedat Quad (89000 Ex3)
ET555/25x Sedat Quad (89000 Ex1)
ET645/30x Sedat Quad (89000 Ex2)
ET470/40x GFP/mCherry ET (89021 Ex2)
ET572/35x GFP/mCherry ET (89021 Ex1)
ET430/24x CFP/YFP (89002 Ex1)
ET500/20x CFP/YFP (89002 Ex2)
Emission Wheel
Empty
Empty
ET455/50m Sedat Quad (89000 Em3)
ET525/36m Sedat Quad (89000 Em2)
ET605/52m Sedat Quad (89000 Em1)
ET705/72m Sedat Quad (89000 Em4)
ET525/50m GFP/mCherry ET (89021 Em2)
ET632/60m GFP/mCherry ET (89021 Em1)
ET470/24m CFP/YFP (89002 Em2)
ET535/30m CFP/YFP (89002 Em1)
Hardware
Nikon Ti-E Microscope
External Phase Unit
Nikon motorized stage
Sutter Lambda XL lamp with filter wheel and shutter
Sutter Emission wheel (wheel C) and brightfield shutter (shutter B) controlled by Lambda 10-3
Coolsnap HQ2 (fluoresence)
Nikon DS-Qi1Mc (external phase)
In Vivo Scientific incubator and CO2 control
Interface Requirements
10-B XL: COM5 (USB)
10-3: COM2
Firmware
Ti: Combination K
TiControl: 4.32
PC
Software