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time_lapse

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Time Lapse Microscope

Optics

Objectives
  1. Plan Fluor 10x/0.3 (Ph1) 654nm/pixel
  2. Plan Apo 20x/0.75 (DIC N2 / 20X) 327nm/pixel
  3. Plan Fluor 40x/0.75 (DIC N2 / 40X I) 164nm/pixel
  4. Plan Fluor 40x/1.3 Oil (DIC N2/ 40X II) 164nm/pixel
  5. Plan Apo 60x/1.2 WI (DIC N2/ 60X II) 109nm/pixel
  6. Plan Apo 100x/1.4 Oil (Ph3) 65.4nm/pixel
Filter Turret
  1. Sedat Quad
  2. GFP/mRFP1
  3. CFP/YFP
  4. FURA2 (Emission Filter within Cube)
  5. D/F/R
  6. Analyzer
Excitation Wheel
  1. ET340x Fura-2 (79000 Ex1)
  2. ET380x Fura-2 (79000 Ex2)
  3. ET402/15x Sedat Quad (89000 Ex4)
  4. ET490/20x Sedat Quad (89000 Ex3)
  5. ET555/25x Sedat Quad (89000 Ex1)
  6. ET645/30x Sedat Quad (89000 Ex2)
  7. ET470/40x GFP/mCherry ET (89021 Ex2)
  8. ET572/35x GFP/mCherry ET (89021 Ex1)
  9. ET430/24x CFP/YFP (89002 Ex1)
  10. ET500/20x CFP/YFP (89002 Ex2)
Emission Wheel
  1. Empty
  2. Empty
  3. ET455/50m Sedat Quad (89000 Em3)
  4. ET525/36m Sedat Quad (89000 Em2)
  5. ET605/52m Sedat Quad (89000 Em1)
  6. ET705/72m Sedat Quad (89000 Em4)
  7. ET525/50m GFP/mCherry ET (89021 Em2)
  8. ET632/60m GFP/mCherry ET (89021 Em1)
  9. ET470/24m CFP/YFP (89002 Em2)
  10. ET535/30m CFP/YFP (89002 Em1)

Hardware

  • Nikon Ti-E Microscope
  • External Phase Unit
  • Nikon motorized stage
  • Sutter Lambda XL lamp with filter wheel and shutter
  • Sutter Emission wheel (wheel C) and brightfield shutter (shutter B) controlled by Lambda 10-3
  • Coolsnap HQ2 (fluoresence)
  • Nikon DS-Qi1Mc (external phase)
  • In Vivo Scientific incubator and CO2 control

Interface Requirements

  • 1 serial port for 10-3
  • Firewire for Coolsnap HQ2
  • 3 USB connectors
  • 10-B XL: COM5 (USB)
  • 10-3: COM2

Firmware

  • Ti: Combination K
  • TiControl: 4.32

PC

  • NIC-HP1
  • Core 2 Duo E8500 (3.16 GHz)
  • 4 GB RAM
  • 64bit Windows 7
  • Radeon HD 3600
  • 500GB Hard drive (RAID 1)

Software

  • NIS-Elements 4.00 (Build 796)
/var/www/html/dokuwiki/data/attic/time_lapse.1344620764.txt.gz · Last modified: 2012/08/10 05:46 (external edit)