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time_lapse [2011/07/19 16:06]
kthorn
time_lapse [2019/01/09 13:18] (current)
dlarsen
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 == Filter Turret == == Filter Turret ==
-  - Sedat Quad+  - Semrock DAPI/​FITC/​TRITC/​Cy5 ​Sedat Quad
   - GFP/mRFP1   - GFP/mRFP1
   - CFP/YFP   - CFP/YFP
-  - 505LP (roGFP)+  - FURA2 (Emission Filter within Cube)
   - D/F/R   - D/F/R
-  - Empty+  - Analyzer
  
-== Excitation ​Wheel ==  +== Excitation ​from Lambda 421 ==  
-  - ET340x ​     Fura-2 +^LED wavelength^Filter in front of LED^ 
-  ET380x ​     Fura-+|340 nm|FF01-340/26-25-STR| 
-  ​ET402/​15x ​  Sedat Quad +|380 nm|FF01-389/38-25-STR| 
-  ET490/20x Sedat Quad +|440 nm|FF01-445/40-25-STR| 
-  ​ET555/​25x Sedat Quad  +|480 nm|FF01-480/40-25-STR| 
-  ET645/30x Sedat Quad +|530 nm|FF01-520/44-25-STR| 
-  ET470/​40x GFP/mCherry ET +|561 nm|FF01-562/40-25-STR| 
-  ET572/​35x GFP/mCherry ET +|630 nm|FF01-630/38-25-STR|
-  ET430/​24x CFP/YFP +
-  ET500/20x CFP/YFP+
  
 == Emission Wheel ==  == Emission Wheel == 
-  - 535/​30m Phil roGFP +  - Fura2 emission filter 
-  - 535/​30m Phil roGFP +  - Empty 
-  - ET455/50m Sedat Quad +  - 440/11m Semrock ​Sedat Quad 
-  - ET525/36m Sedat Quad +  - 525/30m Semrock ​Sedat Quad  
-  - ET605/52m Sedat Quad +  - 607/36m Semrock ​Sedat Quad  
-  - ET705/72m Sedat Quad +  - 684/24m Semrock ​Sedat Quad  
-  - ET525/​50m GFP/​mCherry ET +  - ET525/​50m GFP/​mCherry ET (89021 Em2) 
-  - ET632/​60m GFP/​mCherry ET +  - ET632/​60m GFP/​mCherry ET (89021 Em1) 
-  - ET470/​24m CFP/​YFP +  - ET470/​24m CFP/​YFP ​(89002 Em2) 
-  - ET535/​30m CFP/​YFP+  - ET535/​30m CFP/​YFP ​(89002 Em1)
  
 === Hardware === === Hardware ===
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   * Nikon Ti-E Microscope   * Nikon Ti-E Microscope
   * External Phase Unit   * External Phase Unit
-  * Sutter Lambda ​XL lamp with filter wheel and shutter +  ​* Nikon motorized stage 
-  * Sutter Emission wheel (wheel C) and brightfield shutter (shutter B) controlled by Lambda 10-3 +  ​* Sutter Lambda ​421 LED light source ​with NIDAQ interface 
-  * Coolsnap HQ2 (fluoresence+  * Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM5, 128k Baud Rate) 
-  * Nikon DS-Qi1Mc (external phase)+  * Hamamatsu Flash 4.0 (Right port
 +  * OkoLab incubator and CO2 control
  
 === Interface Requirements === === Interface Requirements ===
  
   * 1 serial port for 10-3   * 1 serial port for 10-3
-  * Firewire for Coolsnap HQ2 
   * 3 USB connectors   * 3 USB connectors
  
Line 67: Line 65:
  
 === PC === === PC ===
-  * NIC-HP1 +  * NIC-HighSpeed2 
-  * Core 2 Duo E8500 (3.16 GHz) +  * Intel Core i7 4770K (3.50 GHz) 
-  * GB RAM+  * 32 GB RAM
   * 64bit Windows 7   * 64bit Windows 7
-  * Radeon HD 3600 +  * 238 GB SSD Hard drive 
-  * 500GB Hard drive (RAID 1)+  * 1 TB SSD Hard drive array (Raid 0) 
 +  * 2 TB Hard drive (RAID 1) 
 +  * NVIDA GeForce GTX 760 
 +  * Active Silicon FireBird Card
  
 === Software === === Software ===
-  * NIS-Elements ​3.22 (Build ​710)+  * NIS-Elements ​4.(Build ​982) 
 + 
 +== Excitation Wheel (filters if you used Lambda XL lamp for earlier experiments) ==  
 +  - ET340x ​     Fura-2 (79000 Ex1) 
 +  - ET380x ​     Fura-2 (79000 Ex2) 
 +  - 387/​11x ​    ​Semrock Sedat Quad  
 +  - 485/​20x Semrock Sedat Quad  
 +  - 560/​25x Semrock Sedat Quad  
 +  - 650/​13x Semrock Sedat Quad  
 +  - ET470/​40x GFP/​mCherry ET (89021 Ex2) 
 +  - ET572/​35x GFP/​mCherry ET (89021 Ex1) 
 +  - ET430/​24x CFP/​YFP (89002 Ex1) 
 +  - ET500/​20x CFP/​YFP (89002 Ex2)
  
/var/www/html/dokuwiki/data/attic/time_lapse.1311116796.txt.gz · Last modified: 2011/07/19 11:06 (external edit)