time_lapse
Differences
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| Both sides previous revision Previous revision Next revision | Previous revision | ||
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time_lapse [2011/06/02 14:48] kthorn |
time_lapse [2019/01/09 13:18] (current) dlarsen |
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| == Filter Turret == | == Filter Turret == | ||
| - | - Sedat Quad | + | - Semrock DAPI/FITC/TRITC/Cy5 Sedat Quad |
| - GFP/mRFP1 | - GFP/mRFP1 | ||
| - CFP/YFP | - CFP/YFP | ||
| - | - 505LP (roGFP) | + | - FURA2 (Emission Filter within Cube) |
| - D/F/R | - D/F/R | ||
| - | - Empty | + | - Analyzer |
| - | == Excitation Wheel == | + | == Excitation from Lambda 421 == |
| - | - 390/22x Phil roGFP | + | ^LED wavelength^Filter in front of LED^ |
| - | - 485/15x Phil roGFP | + | |340 nm|FF01-340/26-25-STR| |
| - | - ET402/15x Sedat Quad | + | |380 nm|FF01-389/38-25-STR| |
| - | - ET490/20x Sedat Quad | + | |440 nm|FF01-445/40-25-STR| |
| - | - ET555/25x Sedat Quad | + | |480 nm|FF01-480/40-25-STR| |
| - | - ET645/30x Sedat Quad | + | |530 nm|FF01-520/44-25-STR| |
| - | - ET470/40x GFP/mCherry ET | + | |561 nm|FF01-562/40-25-STR| |
| - | - ET572/35x GFP/mCherry ET | + | |630 nm|FF01-630/38-25-STR| |
| - | - ET430/24x CFP/YFP | + | |
| - | - ET500/20x CFP/YFP | + | |
| == Emission Wheel == | == Emission Wheel == | ||
| - | - 535/30m Phil roGFP | + | - Fura2 emission filter |
| - | - 535/30m Phil roGFP | + | - Empty |
| - | - ET455/50m Sedat Quad | + | - 440/11m Semrock Sedat Quad |
| - | - ET525/36m Sedat Quad | + | - 525/30m Semrock Sedat Quad |
| - | - ET605/52m Sedat Quad | + | - 607/36m Semrock Sedat Quad |
| - | - ET705/72m Sedat Quad | + | - 684/24m Semrock Sedat Quad |
| - | - ET525/50m GFP/mCherry ET | + | - ET525/50m GFP/mCherry ET (89021 Em2) |
| - | - ET632/60m GFP/mCherry ET | + | - ET632/60m GFP/mCherry ET (89021 Em1) |
| - | - ET470/24m CFP/YFP | + | - ET470/24m CFP/YFP (89002 Em2) |
| - | - ET535/30m CFP/YFP | + | - ET535/30m CFP/YFP (89002 Em1) |
| === Hardware === | === Hardware === | ||
| Line 48: | Line 46: | ||
| * Nikon Ti-E Microscope | * Nikon Ti-E Microscope | ||
| * External Phase Unit | * External Phase Unit | ||
| - | * Sutter Lambda XL lamp with filter wheel and shutter | + | * Nikon motorized stage |
| - | * Sutter Emission wheel (wheel C) and brightfield shutter (shutter B) controlled by Lambda 10-3 | + | * Sutter Lambda 421 LED light source with NIDAQ interface |
| - | * Coolsnap HQ2 (fluoresence) | + | * Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM5, 128k Baud Rate) |
| - | * Nikon DS-Qi1Mc (external phase) | + | * Hamamatsu Flash 4.0 (Right port) |
| + | * OkoLab incubator and CO2 control | ||
| === Interface Requirements === | === Interface Requirements === | ||
| * 1 serial port for 10-3 | * 1 serial port for 10-3 | ||
| - | * Firewire for Coolsnap HQ2 | ||
| * 3 USB connectors | * 3 USB connectors | ||
| Line 67: | Line 65: | ||
| === PC === | === PC === | ||
| - | * NIC-HP1 | + | * NIC-HighSpeed2 |
| - | * Core 2 Duo E8500 (3.16 GHz) | + | * Intel Core i7 4770K (3.50 GHz) |
| - | * 4 GB RAM | + | * 32 GB RAM |
| * 64bit Windows 7 | * 64bit Windows 7 | ||
| - | * Radeon HD 3600 | + | * 238 GB SSD Hard drive |
| - | * 500GB Hard drive (RAID 1) | + | * 1 TB SSD Hard drive array (Raid 0) |
| + | * 2 TB Hard drive (RAID 1) | ||
| + | * NVIDA GeForce GTX 760 | ||
| + | * Active Silicon FireBird Card | ||
| === Software === | === Software === | ||
| - | * NIS-Elements 3.22 (Build 710) | + | * NIS-Elements 4.2 (Build 982) |
| + | |||
| + | == Excitation Wheel (filters if you used Lambda XL lamp for earlier experiments) == | ||
| + | - ET340x Fura-2 (79000 Ex1) | ||
| + | - ET380x Fura-2 (79000 Ex2) | ||
| + | - 387/11x Semrock Sedat Quad | ||
| + | - 485/20x Semrock Sedat Quad | ||
| + | - 560/25x Semrock Sedat Quad | ||
| + | - 650/13x Semrock Sedat Quad | ||
| + | - ET470/40x GFP/mCherry ET (89021 Ex2) | ||
| + | - ET572/35x GFP/mCherry ET (89021 Ex1) | ||
| + | - ET430/24x CFP/YFP (89002 Ex1) | ||
| + | - ET500/20x CFP/YFP (89002 Ex2) | ||
/var/www/html/dokuwiki/data/attic/time_lapse.1307051303.txt.gz · Last modified: 2011/06/02 09:48 (external edit)
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