time_lapse
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision Next revision | Previous revision Last revision Both sides next revision | ||
|
time_lapse [2013/03/20 17:10] dlarsen |
time_lapse [2017/11/27 13:27] dlarsen |
||
|---|---|---|---|
| Line 13: | Line 13: | ||
| == Filter Turret == | == Filter Turret == | ||
| - | - Sedat Quad | + | - Semrock DAPI/FITC/TRITC/Cy5 Sedat Quad |
| - GFP/mRFP1 | - GFP/mRFP1 | ||
| - CFP/YFP | - CFP/YFP | ||
| Line 20: | Line 20: | ||
| - Analyzer | - Analyzer | ||
| - | == Excitation Wheel == | + | == Excitation from Lambda 421 == |
| - | - ET340x Fura-2 (79000 Ex1) | + | ^LED wavelength^Filter in front of LED^ |
| - | - ET380x Fura-2 (79000 Ex2) | + | |340 nm|FF01-340/26-25-STR| |
| - | - ET402/15x Sedat Quad (89000 Ex4) | + | |380 nm|FF01-389/38-25-STR| |
| - | - ET490/20x Sedat Quad (89000 Ex3) | + | |440 nm|FF01-445/40-25-STR| |
| - | - ET555/25x Sedat Quad (89000 Ex1) | + | |480 nm|FF01-480/40-25-STR| |
| - | - ET645/30x Sedat Quad (89000 Ex2) | + | |530 nm|FF01-520/44-25-STR| |
| - | - ET470/40x GFP/mCherry ET (89021 Ex2) | + | |561 nm|FF01-562/40-25-STR| |
| - | - ET572/35x GFP/mCherry ET (89021 Ex1) | + | |630 nm|FF01-630/38-25-STR| |
| - | - ET430/24x CFP/YFP (89002 Ex1) | + | |
| - | - ET500/20x CFP/YFP (89002 Ex2) | + | |
| == Emission Wheel == | == Emission Wheel == | ||
| + | - Fura2 emission filter | ||
| - Empty | - Empty | ||
| - | - Empty | + | - 440/11m Semrock Sedat Quad |
| - | - ET455/50m Sedat Quad (89000 Em3) | + | - 525/30m Semrock Sedat Quad |
| - | - ET525/36m Sedat Quad (89000 Em2) | + | - 607/36m Semrock Sedat Quad |
| - | - ET605/52m Sedat Quad (89000 Em1) | + | - 684/24m Semrock Sedat Quad |
| - | - ET705/72m Sedat Quad (89000 Em4) | + | |
| - ET525/50m GFP/mCherry ET (89021 Em2) | - ET525/50m GFP/mCherry ET (89021 Em2) | ||
| - ET632/60m GFP/mCherry ET (89021 Em1) | - ET632/60m GFP/mCherry ET (89021 Em1) | ||
| Line 49: | Line 47: | ||
| * External Phase Unit | * External Phase Unit | ||
| * Nikon motorized stage | * Nikon motorized stage | ||
| - | * Sutter Lambda XL lamp with filter wheel and shutter | + | * Sutter Lambda 421 LED light source with NIDAQ interface |
| - | * Sutter Emission wheel (wheel C) and brightfield shutter (shutter B) controlled by Lambda 10-3 | + | * Sutter Emission wheel (wheel C) controlled by Lambda 10-B (COM5, 128k Baud Rate) |
| - | * Coolsnap HQ2 (fluoresence) | + | * Coolsnap HQ2 (Left port) |
| - | * Nikon DS-Qi1Mc (external phase) | + | * Hamamatsu Flash 4.0 (Right port) |
| * In Vivo Scientific incubator and CO2 control | * In Vivo Scientific incubator and CO2 control | ||
| Line 69: | Line 67: | ||
| === PC === | === PC === | ||
| - | * NIC-HP1 | + | * NIC-HighSpeed2 |
| - | * Core 2 Duo E8500 (3.16 GHz) | + | * Intel Core i7 4770K (3.50 GHz) |
| - | * 4 GB RAM | + | * 32 GB RAM |
| * 64bit Windows 7 | * 64bit Windows 7 | ||
| - | * Radeon HD 3600 | + | * 238 GB SSD Hard drive |
| - | * 500GB Hard drive (RAID 1) | + | * 1 TB SSD Hard drive array (Raid 0) |
| + | * 2 TB Hard drive (RAID 1) | ||
| + | * NVIDA GeForce GTX 760 | ||
| + | * Active Silicon FireBird Card | ||
| === Software === | === Software === | ||
| - | * NIS-Elements 4.12 (Build 888) | + | * NIS-Elements 4.2 (Build 982) |
| + | |||
| + | == Excitation Wheel (filters if you used Lambda XL lamp for earlier experiments) == | ||
| + | - ET340x Fura-2 (79000 Ex1) | ||
| + | - ET380x Fura-2 (79000 Ex2) | ||
| + | - 387/11x Semrock Sedat Quad | ||
| + | - 485/20x Semrock Sedat Quad | ||
| + | - 560/25x Semrock Sedat Quad | ||
| + | - 650/13x Semrock Sedat Quad | ||
| + | - ET470/40x GFP/mCherry ET (89021 Ex2) | ||
| + | - ET572/35x GFP/mCherry ET (89021 Ex1) | ||
| + | - ET430/24x CFP/YFP (89002 Ex1) | ||
| + | - ET500/20x CFP/YFP (89002 Ex2) | ||
/var/www/html/dokuwiki/data/pages/time_lapse.txt · Last modified: 2019/01/09 13:18 by dlarsen
Page Tools
Except where otherwise noted, content on this wiki is licensed under the following license: CC Attribution 3.0 Unported
