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storm [2015/04/01 12:14]
kthorn
storm [2016/06/23 12:24] (current)
kthorn
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 In order to collect good STORM imaging data sample prep is key. Below are important aspects to optimize and consider. In order to collect good STORM imaging data sample prep is key. Below are important aspects to optimize and consider.
-  * Fixation protocol. Proper fixation to preserve sample ultrastructure is critical for good super-resolution imaging. A recent paper characterized the effect of different fixation protocols on STORM image quality and has optimized protocols for the best image quality. See Whelan et al. 2015.+  * Fixation protocol. Proper fixation to preserve sample ultrastructure is critical for good super-resolution imaging. A recent paper characterized the effect of different fixation protocols on STORM image quality and has optimized protocols for the best image quality. See [[http://​www.nature.com/​articles/​srep07924|Whelan et al. 2015]].
  
   * Signal-to-Noise Ratio: High background and/or weak signal makes it significantly harder to obtain molecule localizations.   * Signal-to-Noise Ratio: High background and/or weak signal makes it significantly harder to obtain molecule localizations.
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 ===== Protocols ===== ===== Protocols =====
-  * [[http://​craterlake.ucsf.edu:​18080/​cm/​wiki/?​id=359|STORM protocols from the Huang lab]] 
-  * [[http://​www.microscopy.med.ualberta.ca/​techniques/​storm-immunofluorescence-protocol/​|A protocol from the Cell Imaging Center at U. Alberta]] 
   * {{:​nikon_storm_sample_preparation.pdf|Nikon N-STORM sample preparation manual}}   * {{:​nikon_storm_sample_preparation.pdf|Nikon N-STORM sample preparation manual}}
   * [[http://​www.leica-microsystems.com/​science-lab/​sample-preparation-for-gsdim-localization-microscopy-protocols-and-tips/​|These protocols for GSDIM sample prep from Leica may also be useful.]]   * [[http://​www.leica-microsystems.com/​science-lab/​sample-preparation-for-gsdim-localization-microscopy-protocols-and-tips/​|These protocols for GSDIM sample prep from Leica may also be useful.]]
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 A nice review of photoactivatible fluorescent proteins for localization microscopy as of 2009. A nice review of photoactivatible fluorescent proteins for localization microscopy as of 2009.
  
-{{::​sci_rep_2014_whelan_dr.pdf|Whelan DR, Bell TD. Image artifacts in Single Molecule Localization Microscopy: why optimization of sample preparation protocols matters. Scientific Reports. 2015 . 10.1038/​srep07924}}+{{::​sci_rep_2014_whelan_dr.pdf|Whelan DR, Bell TD. Image artifacts in Single Molecule Localization Microscopy: why optimization of sample preparation protocols matters. Scientific Reports. 2015 . 10.1038/​srep07924.}} A critical exploration of how sample preparation affects image quality. Has optimized fixation protocols for STORM imaging.
  
 ==== Single dye imaging ==== ==== Single dye imaging ====
/var/www/html/dokuwiki/data/attic/storm.1427915690.txt.gz · Last modified: 2015/04/01 12:14 by kthorn