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spinning_disk_user_guide [2011/08/29 17:06]
kthorn created
spinning_disk_user_guide [2011/09/02 13:15] (current)
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 In general I think it should be safe to always use the '​Fast-Confocal'​ mode, but this has not been extensively tested. ​ If you do use this mode the scope should always try and run as fast as it can.  For instance, if you use the '​Channels'​ group while in '​Fast-Confocal'​ mode, it won't be able to do fast multi-color acquisition,​ because it has to move the filter wheel, but it will still do fast Z-stack acquisition if you select '​Slices first'​. In general I think it should be safe to always use the '​Fast-Confocal'​ mode, but this has not been extensively tested. ​ If you do use this mode the scope should always try and run as fast as it can.  For instance, if you use the '​Channels'​ group while in '​Fast-Confocal'​ mode, it won't be able to do fast multi-color acquisition,​ because it has to move the filter wheel, but it will still do fast Z-stack acquisition if you select '​Slices first'​.
  
-When using the Fast-Confocal channel, there is a single multi-pass emission filter in the detection pathway. ​ This means that the dye selectivity only comes from switching the laser line.  As a result, you may see crosstalk between the channels. ​ In particular, crosstalk between mCherry and GFP has been reported.+When using the 'Fast-Channel'​ channels, there is a single multi-pass emission filter in the detection pathway. ​ This means that the dye selectivity only comes from switching the laser line.  As a result, you may see crosstalk between the channels. ​ In particular, crosstalk between mCherry and GFP has been reported.
/var/www/html/dokuwiki/data/pages/spinning_disk_user_guide.txt · Last modified: 2011/09/02 13:15 (external edit)