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sim_prep [2013/03/22 18:07]
kthorn created
sim_prep [2013/03/22 13:20] (current)
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 Structured Illumination (SIM) imaging is extremely sensitive to spherical aberration. To get the highest quality images (or even to get proper reconstruction),​ you need to minimize spherical aberration. ​ This means: Structured Illumination (SIM) imaging is extremely sensitive to spherical aberration. To get the highest quality images (or even to get proper reconstruction),​ you need to minimize spherical aberration. ​ This means:
  
-  * You should use high precision 0.17 mm coverslips+  * You should use [[coverslips|high precision 0.17 mm coverslips]]
   * You should mount in an appropriate mounting medium. Nikon recommends mounting either in [[http://​www.ncbi.nlm.nih.gov/​pubmed/​17131355|2,​2-thiodiethanol]] or in [[http://​www.invitrogen.com/​site/​us/​en/​home/​brands/​Molecular-Probes/​Key-Molecular-Probes-Products/​ProLong-Antifades-Brand-Page.html|Prolong Gold]] (for a hard-set mounting medium). If using Prolong Gold, be aware of potential shrinkage when it cures.   * You should mount in an appropriate mounting medium. Nikon recommends mounting either in [[http://​www.ncbi.nlm.nih.gov/​pubmed/​17131355|2,​2-thiodiethanol]] or in [[http://​www.invitrogen.com/​site/​us/​en/​home/​brands/​Molecular-Probes/​Key-Molecular-Probes-Products/​ProLong-Antifades-Brand-Page.html|Prolong Gold]] (for a hard-set mounting medium). If using Prolong Gold, be aware of potential shrinkage when it cures.
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