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omx-sr [2017/07/19 08:52]
dlarsen
omx-sr [2017/07/19 08:56] (current)
dlarsen
Line 37: Line 37:
   * You should have your sample as close to the coverslip as possible (e.g. cells grown on coverslip not on slide).   * You should have your sample as close to the coverslip as possible (e.g. cells grown on coverslip not on slide).
   * You should use bright and stable fluorophores.   * You should use bright and stable fluorophores.
-  * You should select the correct immersion oil for you sample. You can use the [[http://​www.gelifesciences.com/​webapp/​wcs/​stores/​servlet/​catalog/​en/​GELifeSciences-us/​service-and-support/​immersion-oil-calculator-web-app#​|GE immersion oil calculator]] to determine the best oil to start with.+  * You should select the correct immersion oil for you sample. You can use the [[http://​www.gelifesciences.com/​webapp/​wcs/​stores/​servlet/​catalog/​en/​GELifeSciences-us/​service-and-support/​immersion-oil-calculator-web-app#​|GE immersion oil calculator]] to determine the best oil to start with. The also have the calculator as a phone app.
  
 As a general rule, if the sample doesn'​t look good in widefield or confocal, SIM imaging will not improve it. You should make every attempt to optimize your staining and reduce background from out-of-focus fluorescence for the best results. ​ As a general rule, if the sample doesn'​t look good in widefield or confocal, SIM imaging will not improve it. You should make every attempt to optimize your staining and reduce background from out-of-focus fluorescence for the best results. ​
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