omx-sr
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omx-sr [2017/07/19 08:56] (current) dlarsen |
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| * You should have your sample as close to the coverslip as possible (e.g. cells grown on coverslip not on slide). | * You should have your sample as close to the coverslip as possible (e.g. cells grown on coverslip not on slide). | ||
| * You should use bright and stable fluorophores. | * You should use bright and stable fluorophores. | ||
| - | * You should select the correct immersion oil for you sample. You can use the [[http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/GELifeSciences-us/service-and-support/immersion-oil-calculator-web-app#|GE immersion oil calculator]] to determine the best oil to start with. | + | * You should select the correct immersion oil for you sample. You can use the [[http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/GELifeSciences-us/service-and-support/immersion-oil-calculator-web-app#|GE immersion oil calculator]] to determine the best oil to start with. The also have the calculator as a phone app. |
| As a general rule, if the sample doesn't look good in widefield or confocal, SIM imaging will not improve it. You should make every attempt to optimize your staining and reduce background from out-of-focus fluorescence for the best results. | As a general rule, if the sample doesn't look good in widefield or confocal, SIM imaging will not improve it. You should make every attempt to optimize your staining and reduce background from out-of-focus fluorescence for the best results. | ||
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