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n-sim

N-SIM Microscope

Optics

Objectives
  1. Plan Apo I 10x/0.45
  2. Plan Fluor ELWD 40x/0.65
  3. Empty
  4. Empty
  5. Plan Apo IR 60x/1.27 Water
  6. Apo TIRF 100x/1.49 Oil
Filter Turret 1
  1. Empty
  2. Empty
  3. Analyzer
  4. UV-2A
  5. FITC
  6. HYQ TRITC
Filter Turret 2
  1. 488
  2. 405
  3. 561
  4. Empty
  5. H/M
  6. 647
SIM Gratings
  • 3D EX V-G 100X/1.49 (for 100x objective, 405nm - 561nm)
  • 3D EX V-R 100x/1.49 (for 100x objective, 405nm - 640nm)
  • 3D EX V-R 60x/1.27 - TIRF 488 100x/1.49 (for 60x objective, 405nm - 640nm or 100x objective, 488nm TIRF)
  • TIRF561 100x/1.49
  • 3D 1layer 100x/1.49
  • 3D 1layer 60x/1.27 - TIRF488 100x/1.49

Hardware

  • Nikon Ti-E Microscope
  • Nikon motorized stage with piezo Z insert
  • Andor DU897 on right port
  • Nikon LUSU laser controller
  • Nikon Intensilight

Interface Requirements

Firmware

  • Ti-E: v4.01
  • HUB-AM: v3.20
  • HUB-AS: v4.00
  • Remote: v1.25
  • Ti-JOY: v1.20

PC

  • Xeon W3670 (3.2GHz)
  • 24 GB RAM
  • 64 bit Windows 7
  • 2 TB Hard drive
  • NVidia Quadro Pro 600

Software

  • NIS-Elements 4.12
/var/www/html/dokuwiki/data/pages/n-sim.txt · Last modified: 2013/03/27 10:14 (external edit)