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Huygens is an easy-to-use commercial deconvolution program. We have it installed on the big deconvolution workstation (The left computer in the lab) and on the left of the two computers in the office.

Their documentation wiki is here; the username to access it is UCSF_NIC and the password is Objective3.

The Huygens Essential Workshop Guide has some useful tutorials and information in it.

If you are doing deconvolution on images acquired on the Spinning Disk here is your backprojected Pinhole Radius and your backprojected Pinhole Spacing by Magnification.

Magnification Radius Spacing Distance
100X 250nm 2.53um
60X 416.7nm 4.22um
40X 625nm 6.22um
20X 1250nm 12.7um
10X 2500nm 12.7um
/var/www/html/dokuwiki/data/attic/huygens/huygens.1319068365.txt.gz · Last modified: 2011/10/19 11:52 (external edit)