Huygens is an easy-to-use commercial deconvolution program. We have it installed on the big deconvolution workstation (The left computer in the lab) and on the left of the two computers in the office.

Their documentation wiki is here; the username to access it is UCSF_NIC and the password is Objective3.

The Huygens Essential Workshop Guide has some useful tutorials and information in it.

If you are doing deconvolution on images acquired on the Spinning Disk here is your backprojected Pinhole Radius and your backprojected Pinhole Spacing by Magnification for the CSU-22.

Here is your backprojected Pinhole Radius and your backprojected Pinhole Spacing by Magnification for the CSU-W1.