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fluorescent_proteins [2012/09/17 17:48]
kthorn
fluorescent_proteins [2017/01/18 14:05] (current)
kthorn
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 ==== Table of Fluorescent Proteins ==== ==== Table of Fluorescent Proteins ====
 +
 +**This page is no longer being maintained. The [[http://​www.fpvis.org/​|interactive graph of fluorescent protein properties]] and [[http://​www.fpvis.org/​PSFP.html|interactive graph of photoswitchable fluorescent protein properties]] pages should be viewed instead.**
  
 === Blue/UV Proteins === === Blue/UV Proteins ===
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 ^ Protein ^ λ<​sub>​ex</​sub>​ ^ λ<​sub>​em</​sub>​ ^ Extinction coeff. ^ QY ^ Brightness ^ Aggregation ^ pKa ^ Source ^ Notes ^ ^ Protein ^ λ<​sub>​ex</​sub>​ ^ λ<​sub>​em</​sub>​ ^ Extinction coeff. ^ QY ^ Brightness ^ Aggregation ^ pKa ^ Source ^ Notes ^
 | TagBFP |  402  |  457  |  52000  |  0.63  |  32.8  | Monomer |  2.7  | [[http://​www.evrogen.com|Evrogen]]| aka mTagBFP; from [27] | | TagBFP |  402  |  457  |  52000  |  0.63  |  32.8  | Monomer |  2.7  | [[http://​www.evrogen.com|Evrogen]]| aka mTagBFP; from [27] |
 +| mTagBFP2 |  399  |  454  |  50600  |  0.64  |  32.4  | Monomer | 2.7 | [48] | |
 | Azurite |  383  |  450  |  26200  |  0.55  |  14.4  | Monomer |  5.0  | [28] | | | Azurite |  383  |  450  |  26200  |  0.55  |  14.4  | Monomer |  5.0  | [28] | |
 | EBFP2 |  383  |  448  |  32000  |  0.56  |  18  | Monomer |  5.3  | [29] | | | EBFP2 |  383  |  448  |  32000  |  0.56  |  18  | Monomer |  5.3  | [29] | |
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 | mWasabi |  493  |  509  |  70000  |  0.80  |  56.0  | Monomer |  6.0  | [[http://​www.allelebiotech.com|Allele Biotech]] | | | mWasabi |  493  |  509  |  70000  |  0.80  |  56.0  | Monomer |  6.0  | [[http://​www.allelebiotech.com|Allele Biotech]] | |
 | Clover |   ​505 ​ |  515  |  111000 ​ |  0.76  |  84.4  | Monomer |  6.1  | [46] | | | Clover |   ​505 ​ |  515  |  111000 ​ |  0.76  |  84.4  | Monomer |  6.1  | [46] | |
 +| mNeonGreen |  506  |  517  |  116000 ​ |  0.80  |  92.8  | Monomer |  5.7  | [49] |[[http://​www.allelebiotech.com/​mneongreen/​|Allele Biotech]] |
  
  
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 | LSS-mKate1 |  463  |  624  |  31200  |  0.08  |  2.5  | Monomer |  3.2  | [32] | | | LSS-mKate1 |  463  |  624  |  31200  |  0.08  |  2.5  | Monomer |  3.2  | [32] | |
 | LSS-mKate2 |  460  |  605  |  26000  |  0.17  |  4.4  | Monomer |  2.7  | [32] | | | LSS-mKate2 |  460  |  605  |  26000  |  0.17  |  4.4  | Monomer |  2.7  | [32] | |
 +| mBeRFP |  446  |  611  |  65000  |  0.27  |  17.6  | Monomer |  5.6  | [50] | |
  
  
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 47. Goedhart, J. et al., Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/​ncomms1738. 47. Goedhart, J. et al., Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/​ncomms1738.
  
 +48. Subach, OM. et al, An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. PLoS One. 2011;​6(12):​e28674. ​
 +
 +49. Shaner, NC. et al, A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat. Meth. 2013 Mar 24.  doi: 10.1038/​nmeth.2413
 +
 +50. Yang, J. et al, mBeRFP, an Improved Large Stokes Shift Red  Fluorescent Protein. PLOS One 2013; 8(6):e64849 doi: 10.1371/​journal.pone.0064849
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