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flatfieldimageacquisition [2014/04/01 11:37]
kthorn
flatfieldimageacquisition [2014/04/01 11:39] (current)
kthorn
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 ==== Data Acquisition and Analysis ==== ==== Data Acquisition and Analysis ====
 +For best results, you should acquire separate flat-field images for each objective and fluorescence channel. In our experience, these images are pretty stable if you don't realign your microscope, so you shouldn'​t have to do it that frequently.
 +
 These instructions assume you're using ImageJ for your analysis. If you're not, it should be straightforward to adapt them to another program. These instructions assume you're using ImageJ for your analysis. If you're not, it should be straightforward to adapt them to another program.
  
/var/www/html/dokuwiki/data/pages/flatfieldimageacquisition.txt · Last modified: 2014/04/01 11:39 by kthorn