User Tools

Site Tools


clearing_methods

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

Both sides previous revision Previous revision
clearing_methods [2013/10/25 16:49]
kthorn
clearing_methods [2016/01/11 09:50]
kthorn
Line 3: Line 3:
 Imaging of thick (>50 um) tissue sections is often improved by clearing of the samples - treating them so that the normally opaque sample becomes clear. ​ In 2007, we tested a number of clearing methods in conjunction with Nan Tang.  The results are summarized in this {{:​mounting_media_tests.pdf|white paper}}. Since then, other clearing methods have been published: Imaging of thick (>50 um) tissue sections is often improved by clearing of the samples - treating them so that the normally opaque sample becomes clear. ​ In 2007, we tested a number of clearing methods in conjunction with Nan Tang.  The results are summarized in this {{:​mounting_media_tests.pdf|white paper}}. Since then, other clearing methods have been published:
  
 +  * [[http://​www.sciencedirect.com/​science/​article/​pii/​S0092867414009313|Passive Clarity (PACT)]] clearing has been used successfully by a number of UCSF labs.
   * The [[http://​www.ncbi.nlm.nih.gov/​pubmed/​21878933|Scale protocol]] has been published by the Miyawaki lab, which uses 4M urea to render whole mouse embryos essentially transparent.   * The [[http://​www.ncbi.nlm.nih.gov/​pubmed/​21878933|Scale protocol]] has been published by the Miyawaki lab, which uses 4M urea to render whole mouse embryos essentially transparent.
   * A [[http://​www.plosone.org/​article/​info%3Adoi%2F10.1371%2Fjournal.pone.0033916|recent paper]] shows that dehydration with tetrahydrofuran and clearing with dibenzyl ether preserves GFP fluorescence better than ethanol dehydration and clearing with BABB.   * A [[http://​www.plosone.org/​article/​info%3Adoi%2F10.1371%2Fjournal.pone.0033916|recent paper]] shows that dehydration with tetrahydrofuran and clearing with dibenzyl ether preserves GFP fluorescence better than ethanol dehydration and clearing with BABB.
/var/www/html/dokuwiki/data/pages/clearing_methods.txt · Last modified: 2016/01/11 09:50 by kthorn