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az100 [2016/12/12 15:11]
kthorn
az100 [2016/12/12 15:51] (current)
kthorn
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   * In general, the 5mm slit provides the best balance of light sheet width and taper. The smaller slits result in thicker light sheets with little benefit, unless you are using the 1x objective with a low zoom.   * In general, the 5mm slit provides the best balance of light sheet width and taper. The smaller slits result in thicker light sheets with little benefit, unless you are using the 1x objective with a low zoom.
   * Don't use zooms less than 2x unless absolutely necessary. There are substantial aberrations at the edges of field of view at low zooms.   * Don't use zooms less than 2x unless absolutely necessary. There are substantial aberrations at the edges of field of view at low zooms.
-  * At and below 3x zoom, set the CameraCrop parameter to 2048 x 2048 - this eliminates aberrations present at the edges of the field of view at these lower magnifications.+  * At 3x zoom and below, set the CameraCrop parameter to 2048 x 2048 - this eliminates aberrations present at the edges of the field of view at these lower magnifications.
   * With the 5x objective, you may need to adjust the AZ100 Z position in steps as small as 2 um to get an accurate focus.   * With the 5x objective, you may need to adjust the AZ100 Z position in steps as small as 2 um to get an accurate focus.
  
/var/www/html/dokuwiki/data/pages/az100.txt · Last modified: 2016/12/12 15:51 by kthorn