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6d

6D Microscope

Optics

Objectives
  1. Plan Apo 4x/0.2
  2. Plan Apo 10x/0.45
  3. Plan Apo 20x/0.75
  4. Plan Apo 40x/0.95 Corr
  5. Plan Apo VC 60x/1.4 Oil
  6. Plan Apo VC 100x/1.4 Oil (DIC N2 / 100X I) 65.4nm/pixel

(other objectives)

  • Plan Apo 2x/0.1
  • Plan Fluor 10x/0.3
  • Super Fluor 40x/1.3 oil
  • Super Fluor 100x/0.5-1.3 iris
Filter Turret
  1. Analyzer
  2. Empty
  3. Empty
  4. CFP/YFP (89002)
  5. GFP/mCherry ET (89021)
  6. Semrock 5-band FF408/504/581/667/762 (DA/FI/TR/Cy5/Cy7-5X5M-B-000)
Excitation Wheel
  1. 387/11x Semrock 5-band
  2. 485/20x Semrock 5-band
  3. 560/25x Semrock 5-band
  4. 650/13x Semrock 5-band
  5. 740/13x Semrock 5-band
  6. ET470/40x GFP/mCherry ET (89021 Ex2)
  7. ET572/35x GFP/mCherry ET (89021 Ex1)
  8. ET430/24x CFP/YFP (89002 Ex1)
  9. ET500/20x CFP/YFP (89002 Ex2)
  10. Open
Emission Wheel
  1. Open
  2. 440/40m Semrock 5-band
  3. 525/30m Semrock 5-band
  4. 607/36m Semrock 5-band
  5. 684/24m Semrock 5-band
  6. 809/81m Semrock 5-band
  7. ET525/50m GFP/mCherry ET (89021 Em2)
  8. ET632/60m GFP/mCherry ET (89021 Em1)
  9. ET470/24m CFP/YFP (89002 Em2)
  10. ET535/30m CFP/YFP (89000 Em1)

Hardware

  • Nikon Ti-E Microscope
  • Sutter Lambda LS lamp with filter wheel and shutter
  • Sutter Emission wheel and brightfield shutter
  • Nikon DS-Qi2 (fluorescence)
  • Nikon DS-Ri2 (color)

Interface Requirements

Firmware

PC

  • Core 2 Duo E8500 (3.16GHz)
  • 4 GB RAM
  • NVidia Quadro NVS 290
  • Windows 7 Pro 64bit SP1

Software

  • NIS-Elements 4.30 (Build 1053)
/var/www/html/dokuwiki/data/pages/6d.txt · Last modified: 2015/07/15 11:17 by dlarsen