Nikon Imaging Center at UCSF / QB3 Wiki
This wiki will be used for general information about microscopy and microscopy-related science and technology that may be of interest to both the UCSF community and the broader microscopy community. If you would like to contribute to the wiki, please email kurt.thorn@ucsf.edu to get an account (we have disabled signups due to spam).
Nikon Imaging Center Microscopes
Here is a repository for information about the NIC microscopes, including technical details and information for end users.
Data Analysis Workstations
Microscopy References and Education
Sample Preparation
Data Analysis
Protocols
Commercial Software available at the NIC
NIS-Elements - Nikon's image acquisition and analysis software. The same software we use to run most of the microscopes, this is also an easy-to-use tool for routine data analysis.
MetaMorph - Another program for interactive data analysis.
MATLAB - A popular commercial programming language and interactive environment for data analysis. Useful links to microscopy tools for Matlab are collected here.
Huygens - Huygens is a powerful and easy to use deconvolution program.
Free Software
ImageJ and Variants - One of the most commonly used free image analysis programs. Has a large and active development community.
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CellProfiler - A free, open source image analysis tool designed for analysis of image-based screens.
IOCBioMicroscope - A free, open source Lucy-Richardson deconvolution tool, written in Python.
Farsight Toolikit - Sophisticated packages for image alignment, segmentation, and neuron / vessel tracing, among other things.
Miscellaneous