Paper Roundup – July 2016

  • A review of Raman imaging [1]
  • DNA-PAINT imaging of DNA origami with nanometer resolution [2]
  • A self-assembling icosahedron that can be used as a fluorescence standard in microscopy [3]
  • A split horseradish peroxidase for labelling of protein interactions [4]
  • A 4Pi single molecule switching microscope, with isotropic 20 nm resolution imaging [5]
  • A comparison of fluorescent protein performance in C. elegans [6]
  • Expansion microscopy with RNA FISH [7] and with conventional fluorescent proteins and antibodies [8]
  • A custom system for calcium imaging of freely walking flies [9]
  • Cell tracking software tools [10]
  • An in-incubator Fourier ptychography system for rapid imaging of 6-well plate [11]


  1. M. Cicerone, "Molecular imaging with CARS micro-spectroscopy", Current Opinion in Chemical Biology, vol. 33, pp. 179-185, 2016.
  2. M. Dai, R. Jungmann, and P. Yin, "Optical imaging of individual biomolecules in densely packed clusters", Nature Nanotechnology, vol. 11, pp. 798-807, 2016.
  3. Y. Hsia, J.B. Bale, S. Gonen, D. Shi, W. Sheffler, K.K. Fong, U. Nattermann, C. Xu, P. Huang, R. Ravichandran, S. Yi, T.N. Davis, T. Gonen, N.P. King, and D. Baker, "Design of a hyperstable 60-subunit protein icosahedron", Nature, vol. 535, pp. 136-139, 2016.
  4. J.D. Martell, M. Yamagata, T.J. Deerinck, S. Phan, C.G. Kwa, M.H. Ellisman, J.R. Sanes, and A.Y. Ting, "A split horseradish peroxidase for the detection of intercellular protein–protein interactions and sensitive visualization of synapses", Nature Biotechnology, vol. 34, pp. 774-780, 2016.
  5. F. Huang, G. Sirinakis, E. Allgeyer, L. Schroeder, W. Duim, E. Kromann, T. Phan, F. Rivera-Molina, J. Myers, I. Irnov, M. Lessard, Y. Zhang, M. Handel, C. Jacobs-Wagner, C. Lusk, J. Rothman, D. Toomre, M. Booth, and J. Bewersdorf, "Ultra-High Resolution 3D Imaging of Whole Cells", Cell, vol. 166, pp. 1028-1040, 2016.
  6. J.K. Heppert, D.J. Dickinson, A.M. Pani, C.D. Higgins, A. Steward, J. Ahringer, J.R. Kuhn, and B. Goldstein, "Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system", Molecular Biology of the Cell, vol. 27, pp. 3385-3394, 2016.
  7. F. Chen, A.T. Wassie, A.J. Cote, A. Sinha, S. Alon, S. Asano, E.R. Daugharthy, J. Chang, A. Marblestone, G.M. Church, A. Raj, and E.S. Boyden, "Nanoscale imaging of RNA with expansion microscopy", Nature Methods, vol. 13, pp. 679-684, 2016.
  8. P.W. Tillberg, F. Chen, K.D. Piatkevich, Y. Zhao, C.(. Yu, B.P. English, L. Gao, A. Martorell, H. Suk, F. Yoshida, E.M. DeGennaro, D.H. Roossien, G. Gong, U. Seneviratne, S.R. Tannenbaum, R. Desimone, D. Cai, and E.S. Boyden, "Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies", Nature Biotechnology, vol. 34, pp. 987-992, 2016.
  9. D. Grover, T. Katsuki, and R.J. Greenspan, "Flyception: imaging brain activity in freely walking fruit flies", Nature Methods, vol. 13, pp. 569-572, 2016.
  10. O. Hilsenbeck, M. Schwarzfischer, S. Skylaki, B. Schauberger, P.S. Hoppe, D. Loeffler, K.D. Kokkaliaris, S. Hastreiter, E. Skylaki, A. Filipczyk, M. Strasser, F. Buggenthin, J.S. Feigelman, J. Krumsiek, A.J.J. van den Berg, M. Endele, M. Etzrodt, C. Marr, F.J. Theis, and T. Schroeder, "Software tools for single-cell tracking and quantification of cellular and molecular properties", Nature Biotechnology, vol. 34, pp. 703-706, 2016.
  11. J. Kim, B.M. Henley, C.H. Kim, H.A. Lester, and C. Yang, "Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy", Biomedical Optics Express, vol. 7, pp. 3097, 2016.

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