Paper Roundup – May 2016

  • Labeling of multiple genomic loci in different colors with CRISPRainbow [1]
  • 3D localization super-resolution microscopy over 4 μm using an astigmatic multifocus microscope [2]
  • Contrast and resolution enhancement by using subtraction of an image acquired from a donut beam from one acquired with a gaussian beam [3]
  • A single objective light sheet system using a micro-fabricated 45º mirror [4]
  • A comparison of OCT and OPT for murine embryo imaging [5]
  • Real time imaging of translation [6]
  • A protocol for coverslip cleaning and functionalization for TIRF microscopy [7]
  • A review of single molecule imaging in live cells [8]
  • Making a Bessel light sheet with a slit and an annulus [9]
  • Hyperspectral imaging of quantum dots for multiple particle tracking [10]


  1. H. Ma, L. Tu, A. Naseri, M. Huisman, S. Zhang, D. Grunwald, and T. Pederson, "Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow", Nature Biotechnology, vol. 34, pp. 528-530, 2016.
  2. L. Oudjedi, J. Fiche, S. Abrahamsson, L. Mazenq, A. Lecestre, P. Calmon, A. Cerf, and M. Nöllmann, "Astigmatic multifocus microscopy enables deep 3D super-resolved imaging", Biomedical Optics Express, vol. 7, pp. 2163, 2016.
  3. K. Korobchevskaya, C. Peres, Z. Li, A. Antipov, C.J.R. Sheppard, A. Diaspro, and P. Bianchini, "Intensity Weighted Subtraction Microscopy Approach for Image Contrast and Resolution Enhancement", Scientific Reports, vol. 6, 2016.
  4. M.B.M. Meddens, S. Liu, P.S. Finnegan, T.L. Edwards, C.D. James, and K.A. Lidke, "Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution", Biomedical Optics Express, vol. 7, pp. 2219, 2016.
  5. M. Singh, R. Raghunathan, V. Piazza, A.M. Davis-Loiacono, A. Cable, T.J. Vedakkan, T. Janecek, M.V. Frazier, A. Nair, C. Wu, I.V. Larina, M.E. Dickinson, and K.V. Larin, "Applicability, usability, and limitations of murine embryonic imaging with optical coherence tomography and optical projection tomography", Biomedical Optics Express, vol. 7, pp. 2295, 2016.
  6. C. Wang, B. Han, R. Zhou, and X. Zhuang, "Real-Time Imaging of Translation on Single mRNA Transcripts in Live Cells", Cell, vol. 165, pp. 990-1001, 2016.
  7. E.M. Kudalkar, Y. Deng, T.N. Davis, and C.L. Asbury, "Coverslip Cleaning and Functionalization for Total Internal Reflection Fluorescence Microscopy", Cold Spring Harbor Protocols, vol. 2016, pp. pdb.prot085548, 2016.
  8. J. Yu, "Single-Molecule Studies in Live Cells", Annual Review of Physical Chemistry, vol. 67, pp. 565-585, 2016.
  9. T. Zhao, S.C. Lau, Y. Wang, Y. Su, H. Wang, A. Cheng, K. Herrup, N.Y. Ip, S. Du, and M.M.T. Loy, "Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets", Scientific Reports, vol. 6, 2016.
  10. S. Labrecque, J. Sylvestre, S. Marcet, F. Mangiarini, B. Bourgoin, M. Verhaegen, S. Blais-Ouellette, and P. De Koninck, "Hyperspectral multiplex single-particle tracking of different receptor subtypes labeled with quantum dots in live neurons", Journal of Biomedical Optics, vol. 21, pp. 046008, 2016.

Paper Roundup: April 2016

  • Axial super-resolution using multi-angle TIRF and photobleaching [1]
  • A tool for simulating localization microscopy data [2]
  • Lattice Light Sheet plus PAINT for 3D localization microscopy of large volumes [3]
  • Monomeric streptavidin combined with enzymatic biotinylation as a labeling probe [4]
  • Massively parallel single-molecule FRET measurements with sCMOS cameras [5]
  • A microfluidic light sheet microscope [6]


  1. Y. Fu, P.W. Winter, R. Rojas, V. Wang, M. McAuliffe, and G.H. Patterson, "Axial superresolution via multiangle TIRF microscopy with sequential imaging and photobleaching", Proceedings of the National Academy of Sciences, vol. 113, pp. 4368-4373, 2016.
  2. V. Venkataramani, F. Herrmannsdörfer, M. Heilemann, and T. Kuner, "SuReSim: simulating localization microscopy experiments from ground truth models", Nature Methods, vol. 13, pp. 319-321, 2016.
  3. W.R. Legant, L. Shao, J.B. Grimm, T.A. Brown, D.E. Milkie, B.B. Avants, L.D. Lavis, and E. Betzig, "High-density three-dimensional localization microscopy across large volumes", Nature Methods, vol. 13, pp. 359-365, 2016.
  4. I. Chamma, M. Letellier, C. Butler, B. Tessier, K. Lim, I. Gauthereau, D. Choquet, J. Sibarita, S. Park, M. Sainlos, and O. Thoumine, "Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin", Nature Communications, vol. 7, pp. 10773, 2016.
  5. M.F. Juette, D.S. Terry, M.R. Wasserman, R.B. Altman, Z. Zhou, H. Zhao, and S.C. Blanchard, "Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale", Nature Methods, vol. 13, pp. 341-344, 2016.
  6. P. Paiè, F. Bragheri, A. Bassi, and R. Osellame, "Selective plane illumination microscopy on a chip", Lab Chip, vol. 16, pp. 1556-1560, 2016.