Paper Roundup – June 2015

  • Quantification of cell cycle state for tumor xenografts using in vivo imaging and computational image analysis [1]
  • Automated imaging and analysis of the yeast proteome [2]
  • A review of fluorogenic protein labeling [3]
  • Fluorescent proteins containing multiple copies of an epitope tag as bright immunofluorescence targets [4]
  • Using uptake of multiple dyes to map neuronal projections [5]
  • A review of correlative light and electron microscopy [6]
  • A review of novel fluorescent protein technologies [7]
  • A swept-focus light sheet microscope, using a cylindrical lens to generate a light sheet whose focus is swept through the sample [8]
  • A nice review of different commercial SIM implementations [9]
  • An open-top SPIM system for imaging samples in plates or in microfluidic devices [10]
  • A review of LOV-based reporters for fluorescence imaging [11]
  • A protocol for fixing yeast for single molecule localization microscopy [12]
  • A review of near-infrared fluorescent proteins [13]


  1. D.R. Chittajallu, S. Florian, R.H. Kohler, Y. Iwamoto, J.D. Orth, R. Weissleder, G. Danuser, and T.J. Mitchison, "In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy", Nature Methods, vol. 12, pp. 577-585, 2015.
  2. Y. Chong, J. Koh, H. Friesen, S. Kaluarachchi Duffy, M. Cox, A. Moses, J. Moffat, C. Boone, and B. Andrews, "Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis", Cell, vol. 161, pp. 1413-1424, 2015.
  3. M.P. Bruchez, "Dark dyes–bright complexes: fluorogenic protein labeling", Current Opinion in Chemical Biology, vol. 27, pp. 18-23, 2015.
  4. S. Viswanathan, M.E. Williams, E.B. Bloss, T.J. Stasevich, C.M. Speer, A. Nern, B.D. Pfeiffer, B.M. Hooks, W. Li, B.P. English, T. Tian, G.L. Henry, J.J. Macklin, R. Patel, C.R. Gerfen, X. Zhuang, Y. Wang, G.M. Rubin, and L.L. Looger, "High-performance probes for light and electron microscopy", Nature Methods, vol. 12, pp. 568-576, 2015.
  5. S. Tsuriel, S. Gudes, R.W. Draft, A.M. Binshtok, and J.W. Lichtman, "Multispectral labeling technique to map many neighboring axonal projections in the same tissue", Nature Methods, vol. 12, pp. 547-552, 2015.
  6. P. de Boer, J.P. Hoogenboom, and B.N.G. Giepmans, "Correlated light and electron microscopy: ultrastructure lights up!", Nature Methods, vol. 12, pp. 503-513, 2015.
  7. J.R. Enterina, L. Wu, and R.E. Campbell, "Emerging fluorescent protein technologies", Current Opinion in Chemical Biology, vol. 27, pp. 10-17, 2015.
  8. K. Dean, P. Roudot, E. Welf, G. Danuser, and R. Fiolka, "Deconvolution-free Subcellular Imaging with Axially Swept Light Sheet Microscopy", Biophysical Journal, vol. 108, pp. 2807-2815, 2015.
  9. K. Marno, L. Al’Zoubi, M. Pearson, M. Posch, . McKnight, and A.P. Wheeler, "The evolution of structured illumination microscopy in studies of HIV", Methods, vol. 88, pp. 20-27, 2015.
  10. R. McGorty, H. Liu, D. Kamiyama, Z. Dong, S. Guo, and B. Huang, "Open-top selective plane illumination microscope for conventionally mounted specimens", Optics Express, vol. 23, pp. 16142, 2015.
  11. A.M. Buckley, J. Petersen, A.J. Roe, G.R. Douce, and J.M. Christie, "LOV-based reporters for fluorescence imaging", Current Opinion in Chemical Biology, vol. 27, pp. 39-45, 2015.
  12. C. Kaplan, and H. Ewers, "Optimized sample preparation for single-molecule localization-based superresolution microscopy in yeast", Nature Protocols, vol. 10, pp. 1007-1021, 2015.
  13. D.M. Shcherbakova, M. Baloban, and V.V. Verkhusha, "Near-infrared fluorescent proteins engineered from bacterial phytochromes", Current Opinion in Chemical Biology, vol. 27, pp. 52-63, 2015.