Paper Roundup – February 2015

  • 2,2′-Thiodiethanol as a simple, rapid clearing agent for mouse brains [1]
  • All-optical neurobiology, with optogenetic stimulation and calcium imaging [2]
  • Cell-cycle staging of cell using integrated DNA fluorescence intensity [3]
  • Swept confocally-aligned planar excitation (SCAPE) microscopy, a high-speed light-sheet-like microscopy system for volumetric imaging [4]
  • Some biological applications of STORM imaging: development of the periodic organization of spectrin in neurons [5] and organization of the synaptonemal complex [6]
  • Modifications of the Nikon C1 and C2 confocal scan heads for UV, multiphoton, and FLIM imaging [7]
  • Two new GFPs, hybrids of GFPγ, superfolder GFP, and Clover, with improved brightness and photostability, for yeast tagging [8]


  1. Y. Aoyagi, R. Kawakami, H. Osanai, T. Hibi, and T. Nemoto, "A Rapid Optical Clearing Protocol Using 2,2′-Thiodiethanol for Microscopic Observation of Fixed Mouse Brain", PLOS ONE, vol. 10, pp. e0116280, 2015.
  2. A.M. Packer, L.E. Russell, H.W.P. Dalgleish, and M. Häusser, "Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivo", Nature Methods, vol. 12, pp. 140-146, 2014.
  3. V. Roukos, G. Pegoraro, T.C. Voss, and T. Misteli, "Cell cycle staging of individual cells by fluorescence microscopy", Nature Protocols, vol. 10, pp. 334-348, 2015.
  4. M.B. Bouchard, V. Voleti, C.S. Mendes, C. Lacefield, W.B. Grueber, R.S. Mann, R.M. Bruno, and E.M.C. Hillman, "Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms", Nature Photonics, vol. 9, pp. 113-119, 2015.
  5. G. Zhong, J. He, R. Zhou, D. Lorenzo, H.P. Babcock, V. Bennett, and X. Zhuang, "Developmental mechanism of the periodic membrane skeleton in axons", eLife, vol. 3, 2014.
  6. K. Schücker, T. Holm, C. Franke, M. Sauer, and R. Benavente, "Elucidation of synaptonemal complex organization by super-resolution imaging with isotropic resolution", Proceedings of the National Academy of Sciences, vol. 112, pp. 2029-2033, 2015.
  7. S.W. BOTCHWAY, K.M. SCHERER, S. HOOK, C.D. STUBBS, E. WESTON, R.H. BISBY, and A.W. PARKER, "A series of flexible design adaptations to the Nikon E-C1 and E-C2 confocal microscope systems for UV, multiphoton and FLIM imaging", Journal of Microscopy, vol. 258, pp. 68-78, 2015.
  8. C.J. Slubowski, A.D. Funk, J.M. Roesner, S.M. Paulissen, and L.S. Huang, "Plasmids for C-terminal tagging inSaccharomyces cerevisiaethat contain improved GFP proteins, Envy and Ivy", Yeast, vol. 32, pp. 379-387, 2015.