Paper roundup – January 2015

  • A review of tools for visualizing mRNA localization [1]
  • There is a whole volume of Methods in Molecular Biology devoted to advanced microscopy techniques [2]
  • A lens-free ultra-wide field imaging approach for brightfield pathology imaging [3]
  • A microfluidic device for doing live cell imaging, followed by fixation and STORM labeling and imaging [4]
  • A holographic fiber-bundle illumination system for spatially patterned photoactivation in freely moving mice [5]
  • Performing parallel STED imaging in thousands of spots simultaneously for fast super-resolution imaging [6]
  • A nice review on biological applications of super-resolution microscopy, with an in depth look at pros and cons of the techniques and sample preparation and labeling methods[7]
  • An improved red fluorescent calcium indicator, R-CaMP2 [8]
  • Nature Methods has named light sheet microscopy as their method of the year and has several review articles discussing it [9].
  • A new computational approach to counting molecules in STORM / PALM super-resolution imaging [10]
  • Using stimulated Raman scattering to image protein synthesis and degradation in live mouse tissues [11]
  • Putting a cheap LCD at the back focal plane of the condenser lens of the microscope for programmable control over the illumination [12]
  • Nickel ions quench the triplet state of Cy3 efficiently and greatly reduce its photobleaching rate [13]
  • Expansion microscopy: expanding tissues to allow high resolution measurements with low resolution imaging [14]
  • Optimized protocols for fixing cell for STORM/PALM imaging [15]
  • Genetic targeting of small molecules using HaloTag [16] or SnapTag [17]
  • A good review on brain imaging in small animals [18]

References

  1. A.R. Buxbaum, G. Haimovich, and R.H. Singer, "In the right place at the right time: visualizing and understanding mRNA localization", Nature Reviews Molecular Cell Biology, vol. 16, pp. 95-109, 2014. http://dx.doi.org/10.1038/nrm3918
  2. "Advanced Fluorescence Microscopy", Methods in Molecular Biology, 2015. http://dx.doi.org/10.1007/978-1-4939-2080-8
  3. A. Greenbaum, Y. Zhang, A. Feizi, P. Chung, W. Luo, S.R. Kandukuri, and A. Ozcan, "Wide-field computational imaging of pathology slides using lens-free on-chip microscopy", Science Translational Medicine, vol. 6, pp. 267ra175-267ra175, 2014. http://dx.doi.org/10.1126/scitranslmed.3009850
  4. J. Tam, G.A. Cordier, . Bálint, . Sandoval Álvarez, J.S. Borbely, and M. Lakadamyali, "A Microfluidic Platform for Correlative Live-Cell and Super-Resolution Microscopy", PLoS ONE, vol. 9, pp. e115512, 2014. http://dx.doi.org/10.1371/journal.pone.0115512
  5. V. Szabo, C. Ventalon, V. De Sars, J. Bradley, and V. Emiliani, "Spatially Selective Holographic Photoactivation and Functional Fluorescence Imaging in Freely Behaving Mice with a Fiberscope", Neuron, vol. 84, pp. 1157-1169, 2014. http://dx.doi.org/10.1016/j.neuron.2014.11.005
  6. F. Bergermann, L. Alber, S.J. Sahl, J. Engelhardt, and S.W. Hell, "2000-fold parallelized dual-color STED fluorescence nanoscopy", Optics Express, vol. 23, pp. 211, 2015. http://dx.doi.org/10.1364/OE.23.000211
  7. E.F. Fornasiero, and F. Opazo, "Super-resolution imaging for cell biologists", BioEssays, vol. 37, pp. 436-451, 2015. http://dx.doi.org/10.1002/bies.201400170
  8. M. Inoue, A. Takeuchi, S. Horigane, M. Ohkura, K. Gengyo-Ando, H. Fujii, S. Kamijo, S. Takemoto-Kimura, M. Kano, J. Nakai, K. Kitamura, and H. Bito, "Rational design of a high-affinity, fast, red calcium indicator R-CaMP2", Nature Methods, vol. 12, pp. 64-70, 2014. http://dx.doi.org/10.1038/nmeth.3185
  9. "Method of the Year 2014", Nature Methods, vol. 12, pp. 1-1, 2014. http://dx.doi.org/10.1038/nmeth.3251
  10. G.C. Rollins, J.Y. Shin, C. Bustamante, and S. Pressé, "Stochastic approach to the molecular counting problem in superresolution microscopy", Proceedings of the National Academy of Sciences, vol. 112, pp. E110-E118, 2014. http://dx.doi.org/10.1073/pnas.1408071112
  11. L. Wei, Y. Shen, F. Xu, F. Hu, J.K. Harrington, K.L. Targoff, and W. Min, "Imaging Complex Protein Metabolism in Live Organisms by Stimulated Raman Scattering Microscopy with Isotope Labeling", ACS Chemical Biology, vol. 10, pp. 901-908, 2015. http://dx.doi.org/10.1021/cb500787b
  12. K. Guo, Z. Bian, S. Dong, P. Nanda, Y.M. Wang, and G. Zheng, "Microscopy illumination engineering using a low-cost liquid crystal display", Biomedical Optics Express, vol. 6, pp. 574, 2015. http://dx.doi.org/10.1364/BOE.6.000574
  13. V. Glembockyte, R. Lincoln, and G. Cosa, "Cy3 Photoprotection Mediated by Ni2+for Extended Single-Molecule Imaging: Old Tricks for New Techniques", Journal of the American Chemical Society, vol. 137, pp. 1116-1122, 2015. http://dx.doi.org/10.1021/ja509923e
  14. F. Chen, P.W. Tillberg, and E.S. Boyden, "Expansion microscopy", Science, vol. 347, pp. 543-548, 2015. http://dx.doi.org/10.1126/science.1260088
  15. D.R. Whelan, and T.D.M. Bell, "Image artifacts in Single Molecule Localization Microscopy: why optimization of sample preparation protocols matters", Scientific Reports, vol. 5, 2015. http://dx.doi.org/10.1038/srep07924
  16. D. Li, L. Liu, and W. Li, "Genetic Targeting of a Small Fluorescent Zinc Indicator to Cell Surface for Monitoring Zinc Secretion", ACS Chemical Biology, vol. 10, pp. 1054-1063, 2015. http://dx.doi.org/10.1021/cb5007536
  17. G. Yang, F. de Castro Reis, M. Sundukova, S. Pimpinella, A. Asaro, L. Castaldi, L. Batti, D. Bilbao, L. Reymond, K. Johnsson, and P.A. Heppenstall, "Genetic targeting of chemical indicators in vivo", Nature Methods, vol. 12, pp. 137-139, 2014. http://dx.doi.org/10.1038/nmeth.3207
  18. M.B. Ahrens, and F. Engert, "Large-scale imaging in small brains", Current Opinion in Neurobiology, vol. 32, pp. 78-86, 2015. http://dx.doi.org/10.1016/j.conb.2015.01.007