Fluorobrite DMEM and sptPALM

A number of our users have been testing out the low-fluorescence DMEM from Life Technologies, Fluorobrite DMEM, and many have found that it substantially improves the signal-to-background of their experiments, particularly for imaging dim samples. Recently we tried it when doing sptPALM of PA-GFP, and found that it made a substantial difference in image quality. Below are two movies, taken under identical conditions, and scaled identically, of single PA-GFP labeled receptors diffusing in the membrane of a cell. They are illuminated with modest 488 nm excitation and weak 405 nm excitation to switch PA-GFP on. The movies were acquired by TIRF, and are shown in real time. The top movie is of cells in conventional DMEM-based media; the bottom, cells in Fluorobrite DMEM. The Fluorobrite media substantially improves the image contrast; there’s also a noticeable reduction in background events outside of the cell.

If you’re doing low-signal imaging, particularly in the GFP channel, I think the Fluorobrite media is well worth trying out.

Job Posting: Director of the Gladstone Histology and Light Microscopy Core

First, apologies for the infrequent posting of late. Due to both literal and figurative (a new R01) children, I haven’t had much spare time to post here. I hope this changes in the future.

Second, I’m helping the Gladstone Institutes, across the street from UCSF, recruit a new director for their microscopy and histology core.  The full job ad is below, but briefly, we are looking for a PhD to direct the day to day operations of the core, which would include microscopy training, maintenance, advising users on how best to acquire the data they need, and overseeing the histology operations.

This position has the potential for a lot of freedom and growth; the ideal candidate will play a major role in shaping the imaging resources available at the Gladstone Institutes and UCSF.

For more information and to apply, see the following link: