Since my previous post on flat-field correction, I’ve become aware of two commercial sources for slides with uniform fluorescent films deposited on them: Valley Scientific and Argolight. These are more expensive than the DIY solution but more convenient. The Argolight slide also includes a number of very small features for measurement of resolution and distortion (this also makes it fairly expensive). I don’t have personal experience with either one, but they may come in handy.
I hope to have a report soon on the testing of all the flat-fielding dyes. We need to do more testing, but we have promising initial results on using Acid Blue 9 to calibrate the Cy5 channel.