Paper roundup: December 2013

  • A clever method for acquiring 0.5 gigapixel images by projecting a magnified image of the sample onto a modified flatbed scanner [1]
  • Electroporating small molecule fluorophore-labelled molecules into E. coli for long term imaging [2]
  • A Brainbow-like technique for labeling cells in Drosophila development [3]
  • A review of high speed nonlinear optical microscopy (two-photon, CARS, and similar techniques) [4]
  • Optogenetic control of Ras/Erk signaling [5]
  • Protocols for capillary mounting of spheroids [6] and cysts [7] for light sheet imaging.
  • Resolution doubling using a conventional spinning disk confocal microscope with stroboscopic illumination [8]
  • Using TALEs to target fluorescent proteins to specific genomic loci [9]
  • A review of fluorescent membrane probes [10]
  • A protocol for using spinach-based aptamers for imaging ADP concentration in bacteria [11]

References

  1. G. Zheng, X. Ou, and C. Yang, "05 gigapixel microscopy using a flatbed scanner", Biomedical Optics Express, vol. 5, pp. 1, 2013. http://dx.doi.org/10.1364/BOE.5.000001
  2. R. Crawford, J. Torella, L. Aigrain, A. Plochowietz, K. Gryte, S. Uphoff, and A. Kapanidis, "Long-Lived Intracellular Single-Molecule Fluorescence Using Electroporated Molecules", Biophysical Journal, vol. 105, pp. 2439-2450, 2013. http://dx.doi.org/10.1016/j.bpj.2013.09.057
  3. O. Kanca, E. Caussinus, A.S. Denes, A. Percival-Smith, and M. Affolter, "Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development", Development, vol. 141, pp. 472-480, 2013. http://dx.doi.org/10.1242/dev.102913
  4. P. So, E. Yew, and C. Rowlands, "High-Throughput Nonlinear Optical Microscopy", Biophysical Journal, vol. 105, pp. 2641-2654, 2013. http://dx.doi.org/10.1016/j.bpj.2013.08.051
  5. J. Toettcher, O. Weiner, and W. Lim, "Using Optogenetics to Interrogate the Dynamic Control of Signal Transmission by the Ras/Erk Module", Cell, vol. 155, pp. 1422-1434, 2013. http://dx.doi.org/10.1016/j.cell.2013.11.004
  6. J. Swoger, F. Pampaloni, and E.H. Stelzer, "Imaging Cellular Spheroids with a Single (Selective) Plane Illumination Microscope", Cold Spring Harbor Protocols, vol. 2014, pp. pdb.prot080176, 2013. http://dx.doi.org/10.1101/pdb.prot080176
  7. J. Swoger, F. Pampaloni, and E.H. Stelzer, "Imaging MDCK Cysts with a Single (Selective) Plane Illumination Microscope", Cold Spring Harbor Protocols, vol. 2014, pp. pdb.prot080184, 2013. http://dx.doi.org/10.1101/pdb.prot080184
  8. O. Schulz, C. Pieper, M. Clever, J. Pfaff, A. Ruhlandt, R.H. Kehlenbach, F.S. Wouters, J. Grosshans, G. Bunt, and J. Enderlein, "Resolution doubling in fluorescence microscopy with confocal spinning-disk image scanning microscopy", Proceedings of the National Academy of Sciences, vol. 110, pp. 21000-21005, 2013. http://dx.doi.org/10.1073/pnas.1315858110
  9. H. Ma, P. Reyes-Gutierrez, and T. Pederson, "Visualization of repetitive DNA sequences in human chromosomes with transcription activator-like effectors", Proceedings of the National Academy of Sciences, vol. 110, pp. 21048-21053, 2013. http://dx.doi.org/10.1073/pnas.1319097110
  10. A. Klymchenko, and R. Kreder, "Fluorescent Probes for Lipid Rafts: From Model Membranes to Living Cells", Chemistry & Biology, vol. 21, pp. 97-113, 2014. http://dx.doi.org/10.1016/j.chembiol.2013.11.009
  11. R.L. Strack, W. Song, and S.R. Jaffrey, "Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria", Nature Protocols, vol. 9, pp. 146-155, 2013. http://dx.doi.org/10.1038/nprot.2014.001