Paper Roundup: September 2013

  • A short review of tissue clearing methods. [1]
  • Optimizing FRET probes by driving interaction of the donor and acceptor in a favorable FRET geometry, either by electrostatic interaction or by using weak heterodimerization domains. [2]
  • A review on alternating laser excitation (ALEX) for FRET microscopy. [3]
  • Silicon nanoparticles: a novel bright, nonblinking probe for single molecule studies (essentially a non-blinking Qdot replacement). [4]
  • Fluorescent nanodiamonds have been used for long-term tracking of lung stem cells in mice. [5]
  • A new red-shifted channelrhodopsin. [6]
  • Journal of Optics has a special issue on high-resolution (super-resolution) optical imaging.
  • A comparison of single particle tracking and temporal image correlation spectroscopy for monitoring diffusion and transport. [7]
  • A comprehensive review of fluorescent proteins for super-resolution imaging. [8]
  • SuperNova: a monomeric version of KillerRed for chromophore-assisted laser inactivation. [9]
  • A frequency domain approach for very high speed imaging, where different pixels are excited at different frequencies. The fluorescence emission is recorded on a PMT and signals at different frequencies are extracted to recover the image. It runs at over 4000 fps for 200 x 92 pixels. [10]
  • A wide-field two-photon approach for neuronal imaging in C. elegans. [11]

References

  1. D.A. Yushchenko, and C. Schultz, "Tissue Clearing for Optical Anatomy", Angewandte Chemie International Edition, vol. 52, pp. 10949-10951, 2013. http://dx.doi.org/10.1002/anie.201306039
  2. R. Grünberg, J.V. Burnier, T. Ferrar, V. Beltran-Sastre, F. Stricher, A.M. van der Sloot, R. Garcia-Olivas, A. Mallabiabarrena, X. Sanjuan, T. Zimmermann, and L. Serrano, "Engineering of weak helper interactions for high-efficiency FRET probes", Nature Methods, vol. 10, pp. 1021-1027, 2013. http://dx.doi.org/10.1038/nmeth.2625
  3. J. Hohlbein, T.D. Craggs, and T. Cordes, "Alternating-laser excitation: single-molecule FRET and beyond", Chem. Soc. Rev., vol. 43, pp. 1156-1171, 2014. http://dx.doi.org/10.1039/C3CS60233H
  4. H. Nishimura, K. Ritchie, R.S. Kasai, M. Goto, N. Morone, H. Sugimura, K. Tanaka, I. Sase, A. Yoshimura, Y. Nakano, T.K. Fujiwara, and A. Kusumi, "Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging", The Journal of Cell Biology, vol. 202, pp. 967-983, 2013. http://dx.doi.org/10.1083/jcb.201301053
  5. T. Wu, Y. Tzeng, W. Chang, C. Cheng, Y. Kuo, C. Chien, H. Chang, and J. Yu, "Tracking the engraftment and regenerative capabilities of transplanted lung stem cells using fluorescent nanodiamonds", Nature Nanotechnology, vol. 8, pp. 682-689, 2013. http://dx.doi.org/10.1038/nnano.2013.147
  6. J.Y. Lin, P.M. Knutsen, A. Muller, D. Kleinfeld, and R.Y. Tsien, "ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation", Nature Neuroscience, vol. 16, pp. 1499-1508, 2013. http://dx.doi.org/10.1038/nn.3502
  7. F. LUND, and D. WÜSTNER, "A comparison of single particle tracking and temporal image correlation spectroscopy for quantitative analysis of endosome motility", Journal of Microscopy, vol. 252, pp. 169-188, 2013. http://dx.doi.org/10.1111/jmi.12080
  8. K. Nienhaus, and G. Ulrich Nienhaus, "Fluorescent proteins for live-cell imaging with super-resolution", Chem. Soc. Rev., vol. 43, pp. 1088-1106, 2014. http://dx.doi.org/10.1039/c3cs60171d
  9. K. Takemoto, T. Matsuda, N. Sakai, D. Fu, M. Noda, S. Uchiyama, I. Kotera, Y. Arai, M. Horiuchi, K. Fukui, T. Ayabe, F. Inagaki, H. Suzuki, and T. Nagai, "SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation", Scientific Reports, vol. 3, 2013. http://dx.doi.org/10.1038/srep02629
  10. E.D. Diebold, B.W. Buckley, D.R. Gossett, and B. Jalali, "Digitally synthesized beat frequency multiplexing for sub-millisecond fluorescence microscopy", Nature Photonics, vol. 7, pp. 806-810, 2013. http://dx.doi.org/10.1038/nphoton.2013.245
  11. T. Schrödel, R. Prevedel, K. Aumayr, M. Zimmer, and A. Vaziri, "Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light", Nature Methods, vol. 10, pp. 1013-1020, 2013. http://dx.doi.org/10.1038/nmeth.2637

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