Welcome to ASEdb, the Alanine Scanning Energetics database. ASEdb is a repository for energetics of sidechain interactions determined by alanine-scanning mutagenesis.

ASEdb is a searchable database of binding energy changes reulting from mutations of protein side-chains to alanine. It is no longer being actively maintained, and access may be discontinud in the future. You may want to download the mysql dump of the data to ensure future access to it. If someone would like to take over maintenance of it, please contact me at the address below.

ASEdb is an outgrowth of our earlier paper, "Anatomy of hotspots in protein-protein interfaces". In this paper, we compiled a database of alanine-scanning mutations in protein-protein heterodimers to understand how hotspots of binding energy arise. ASEdb consists of this data, supplemented with subsequently published alanine scanning data. A description of ASEdb has been published in Bioinformatics. You can download the reprint here. If you use this database in any published work, you should cite this paper.