Welcome to ASEdb, the Alanine Scanning Energetics database. ASEdb is a repository for energetics of sidechain interactions determined by alanine-scanning mutagenesis.

ASEdb is a searchable database of binding energy changes reulting from mutations of protein side-chains to alanine. It can also be updated with new data as it is determined.

ASEdb is an outgrowth of our earlier paper, "Anatomy of hotspots in protein-protein interfaces". In this paper, we compiled a database of alanine-scanning mutations in protein-protein heterodimers to understand how hotspots of binding energy arise. ASEdb consists of this data, supplemented with subsequently published alanine scanning data. A description of ASEdb has been published in Bioinformatics. You can download the reprint here. If you use this database in any published work, you should cite this paper.

In addition, we would like to keep the database current. If you have recently published an alanine scan of a protein and measured binding energies to a cognate partner, please add the data to ASEdb. The alanine scan need not be complete -- even a few residues help. However, we do not accept data that has not been published in a peer-reviewed journals (we don't have the resources to validate it ourselves), so please send me a reprint (either by e-mail or snail mail) of the paper from which the data comes.