6D High Throughput Microscope
Nikon Eclipse Ti-E Motorized Inverted Microscope
- NIS-Elements Advanced Research
- Nikon integrated Perfect Focus System
- Nikon Encoded motorized stage
- Photometrics Coolsnap HQ2 Camera top of the line interline CCD camera
- Sutter Lambda DG-4 Ultra high speed wavelegnth switcher (less than 1.2 ms)
- Sutter Lambda 10-B Emission Filter Wheel (as fast as 40 ms for adjacent filters)
- Sutter Smart Shutters (8ms open/close time)
- Chroma Filters
| Objectives: | |||||||
|---|---|---|---|---|---|---|---|
| Objective | Resolution limit (nm) | Pixel Size (nm/px) | Depth of field (um) | Transmitted Light | Comments | ||
| 4x/0.2 air Plan Apo | 2350 | 1635 | 76.9 | n/a | |||
| 10x/0.45 air Plan Apo | 678 | 654 | 6.42 | DIC N1/10x | |||
| 20x/0.75 air Plan Apo | 407 | 455 | 2.31 | DIC N2/20x | |||
| 40x/0.95 air Plan Apo | 321 | 163.5 | 1.44 | DIC N2/40x I | Correction Collar | ||
| 60x/1.40 oil Plan Apo VC | 218 | 109 | 0.66 | DIC N2/60x II | no | ||
| 100x/1.40 oil Plan Apo VC | 218 | 65.4 | 0.66 | DIC N2/100x I | no | ||
| 2x/0.10 air Plan Apo | 3050 | 3270 | 130 | n/a | no | ||
| 10x/0.30 air Plan Fluor | 102 | 654 | 14.4 | Phase 1 | Fura-2 | ||
| 40x/1.30 oil S Fluor | 235 | 163.5 | 0.77 | DIC N2/40x II | Fura-2 | ||
| 100x/0.5-1.3 oil S Fluor | 610 - 235 | 65.4 | 5.2 - 0.77 | n/a | Fura-2 |
||
| Filters: | |||
|---|---|---|---|
| Fluorophore | Excitation | Dichroic | Emission |
| DIC | halogen | 1. Analyzer | 2. open |
| Brightfield | halogen | 2. Open | 2. open |
| Fura-2 | 1. D340xv2 | 3. Fura-2 | 2. open |
| Fura-2 | 2. D380xv2 | 2. open | |
| CFP | 2. ET430/24x | 4. CFP/YFP | 9. WR470/24m |
| YFP | 1. ET500/20x | 10. ET535/30m | |
| GFP | 4. S470/30x | 5. GFP/mRFP1 | 7. S510/30m |
| mRFP1 | 3. S565/50x | 8. S650/75m | |
| DAPI | 4. D350/50x | 6. Sedat Quad | 3. ET455/50m |
| FITC | 3. ET490/20x | 4. ET525/36m | |
| Cy3 | 2. ET555/25x | 5. ET605/52m | |
| Cy5 | 1. ET645/30x | 6. ET705/72m | |
